ERAS 抗体 (N-Term)
Quick Overview for ERAS 抗体 (N-Term) (ABIN2800622)
抗原
See all ERAS 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 51-81, N-Term
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原理
- Rabbit Anti-Human ERAS (N-term) (F66) Antibody
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免疫原
- This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-81 amino acids from the N-terminal region of human ERAS.
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亚型
- Ig Fraction
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anti-ES cell expressed Ras (ERAS) antibody
ERAS 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (C-Term) antibodyERAS 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 19-52), (N-Term) antibodyERAS 适用: 小鼠 WB, FACS 宿主: 兔 Polyclonal RB51538 unconjugated
anti-ES cell expressed Ras (ERAS) (AA 202-236), (C-Term) antibodyERAS 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal RB52380 unconjugated
anti-ES cell expressed Ras (ERAS) (N-Term) antibodyERAS 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 13-42), (N-Term) antibodyERAS 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 3-230) antibodyERAS 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (C-Term) antibodyERAS 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (N-Term) antibodyERAS 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, Bat, 猴, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 143-192) antibodyERAS 适用: 人, 小鼠, 大鼠, Cow, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunofluorescence, Western Blot, Immunohistochemistry
Recommended Dilutions
IF: 1:100, WB: 1:1000, IHC: 1:10-50Fluorescent confocal image of SY5Y cells stained with ERAS (N-term) (F66) antibody. SY5Y cells were fixed with 4 % PFA (20 min), permeabilized with Triton X-100 (0.2 %, 30 min). Cells were then incubated with 102-12613 ERAS (N-term) (F66) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/mL, 5 min). ERAS immunoreactivity is localized to the plasma membrane of SY5Y cells.Western blot analysis of ERAS Antibody (N-term) (F66) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.0 mg/mL
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C (short-term), -20°C (long-term)
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- ERAS (ES cell expressed Ras (ERAS))
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别名
- ERAS
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背景
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Target Description: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.
Gene Symbol: ERAS
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分子量
- 25287 Da
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基因ID
- 3266
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UniProt
- Q7Z444
抗原
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