E2F5 抗体 (N-Term)
Quick Overview for E2F5 抗体 (N-Term) (ABIN2792655)
抗原
See all E2F5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- KAEIEDLELK ERELDQQKLL LQQSIKNVMD DSINNRFSYV THEDICNCFN
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交叉反应
- 小鸡, 人, 小鼠, 大鼠
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预测反应
- Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 84%
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产品特性
- This is a rabbit polyclonal antibody against E2F5. It was validated on Western Blot using a cell lysate as a positive control.
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纯化方法
- Affinity Purified
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免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of human E2F5
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anti-E2F5 (E2F5) (AA 101-200) antibody
E2F5 适用: 人, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-E2F5 (E2F5) (AA 66-93), (N-Term) antibodyE2F5 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB23726 unconjugated
anti-E2F5 (E2F5) antibodyE2F5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E2F5 (E2F5) (AA 193-298) antibodyE2F5 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E2F5 (E2F5) antibodyE2F5 适用: 人 DB, ICC 宿主: 小鼠 Monoclonal 220D1a unconjugated
anti-E2F5 (E2F5) (AA 134-183) antibodyE2F5 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 兔, 斑马鱼, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-E2F5 (E2F5) (AA 197-346) antibodyE2F5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB Suggested Anti-E2F5 Antibody Titration: 0.2-1 µg/mL
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator. -
说明
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Antigen size: 346 AA
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- E2F5
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别名
- E2F5
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背景
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E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.
Alias Symbols: E2F-5
Protein Interaction Partner: RBL2, UBC, KMT2D, BRCC3, SMAD3, EP300, XPO1, CREBBP, TFDP1, RBL1, BIRC5, MYC, MT1G,
Protein Size: 346 -
分子量
- 38 kDa
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基因ID
- 1875
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NCBI登录号
- NM_001951, NP_001942
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UniProt
- Q15329
抗原
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