ARHGEF4 抗体 (N-Term)
(1 validation)Quick Overview for ARHGEF4 抗体 (N-Term) (ABIN2787993)
抗原
See all ARHGEF4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- ADDYEAPRAG AREADDSGPE AQSCKDQMRT NVINEILSTE RDYIKHLRDI
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预测反应
- Cow: 79%, Dog: 93%, Guinea Pig: 92%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%
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产品特性
- This is a rabbit polyclonal antibody against Arhgef4. It was validated on Western Blot.
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纯化方法
- Affinity Purified
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免疫原
- The immunogen is a synthetic peptide directed towards the N-terminal region of Mouse Arhgef4
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anti-rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4) (N-Term) antibody
Verified ARHGEF4 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4) (AA 143-271) antibodyARHGEF4 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal ARHGEF-08 unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4) (AA 143-271) antibody (PE)ARHGEF4 适用: 人 ICFC 宿主: 小鼠 Monoclonal ARHGEF-08 PE
anti-rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4) (AA 1-100) antibodyARHGEF4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4) (Internal Region) antibodyARHGEF4 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4) (AA 51-100) antibodyARHGEF4 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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说明
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Antigen size: 484 AA
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限制
- 仅限研究用
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- by
- Instituto de Parasitología y Biomedicina López-Neyra
- No.
- #100106
- 日期
- 2016.12.23
- 抗原
- ARHGEF4
- Lot Number
- QC30159-41213
- Method validated
- Western Blotting
- Positive Control
- primary mouse microglia
- Negative Control
- Notes
- Passed. ABIN2787993 specifically recognizes Arhgef4 in primary mouse microglia lysates.
- Primary Antibody
- ABIN2787993
- Secondary Antibody
- anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)
- Full Protocol
- Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
- Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, protease inhibitors (Sigma)).
- Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
- Denature 5µg, 10µg and 30µg total protein for 5min at 95°C in 5µl, 10µl and 20µl Laemmli SDS sample buffer and subsequently separate them on a denaturing self-made 10% SDS-PAGE for 1h at 140V.
- Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
- Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk for 1h at RT.
- Wash membrane 3x 5min with TBST.
- Incubation with primary rabbit anti-ARHGEF4 antibody (antibodies-online, ABIN2787993, lot QC30159-41213) diluted 1:500 in TBST containing 2% BSA ON at 4°C.
- Wash membrane 5x 5min with TBST.
- Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
- Wash membrane 5x 5min with TBST and 3x 5min with TBS.
- Reveal protein bands using ECL Plus Western Blotting Detection Reagent (GE Healthcare, RPN2132, lot 4645641) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
- Experimental Notes
- The ARHGEF4 antibody ABIN2787993 reveals multiple bands as is expected for murine Arhgef4.
生效 #100106 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Indicated amounts of total protein from primary mouse microglia cell lysate were separated on a denaturing SDS-PAGE gel and incubated with ABIN2787993 as described in the protocol.]()
Indicated amounts of total protein from primary mouse microglia cell lysate were separated on a denaturing SDS-PAGE gel and incubated with ABIN2787993 as described in the protocol.
Full Methods -
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))
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别名
- Arhgef4
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背景
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Arhgef4 acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion By similarity. It is required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Arhgef4 is involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
Alias Symbols: 9330140K16Rik, Asef, ENSMUSG00000070955
Protein Size: 484 -
分子量
- 57 kDa
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基因ID
- 226970
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NCBI登录号
- NM_183019, NP_898840
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UniProt
- Q7TNR9
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途径
- Neurotrophin Signaling Pathway
抗原
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