GCLM 抗体 (Middle Region)
(2 references)Quick Overview for GCLM 抗体 (Middle Region) (ABIN2785805)
抗原
See all GCLM 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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序列
- KPNSNQVNLA SCCVMPPDLT AFAKQFDIQL LTHNDPKELL SEASFQEALQ
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预测反应
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%
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产品特性
- This is a rabbit polyclonal antibody against GCLM. It was validated on Western Blot using a cell lysate as a positive control.
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纯化方法
- Affinity Purified
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免疫原
- The immunogen is a synthetic peptide directed towards the middle region of human GCLM
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anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 40-251) antibody
GCLM 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 246-274), (C-Term) antibodyGCLM 适用: 人, 小鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB22980 unconjugated
anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274), (Regulatory Subunit) antibodyGCLM 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) antibodyGCLM 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2B8 unconjugated
anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 245-274), (C-Term) antibodyGCLM 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) antibodyGCLM 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (N-Term) antibodyGCLM 适用: 人, 小鼠, 大鼠, Pig WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 182-231) antibodyGCLM 适用: 人, 小鼠, 大鼠, Cow, 兔, 猴, 犬, 豚鼠, 马, 斑马鱼, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) antibodyGCLM 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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说明
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Antigen size: 274 AA
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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: "Molecular mechanisms of lipopolysaccharide-mediated inhibition of glutathione synthesis in mice." in: Free radical biology & medicine, Vol. 68, pp. 148-58, (2014) (PubMed).
: "Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis." in: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 36341-55, (2012) (PubMed).
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: "Molecular mechanisms of lipopolysaccharide-mediated inhibition of glutathione synthesis in mice." in: Free radical biology & medicine, Vol. 68, pp. 148-58, (2014) (PubMed).
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- GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))
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别名
- GCLM
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背景
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Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: GLCLR
Protein Interaction Partner: UBC, NAGK, GLRX3, GNAI2, GCLC, STOX1, IRF7, CALM1, SORD, CUL3,
Protein Size: 274 -
分子量
- 31 kDa
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基因ID
- 2730
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NCBI登录号
- NM_002061, NP_002052
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UniProt
- P48507
抗原
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