CCDC42 抗体 (Middle Region)
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Quick Overview for CCDC42 抗体 (Middle Region) (ABIN2785068)
抗原
See all CCDC42 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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序列
- ENSEFEEIHE VIARYKTLVS MHHDLMQSAQ EGQEKIEHAK ARLARYMEEK
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预测反应
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 93%
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产品特性
- This is a rabbit polyclonal antibody against Ccdc42. It was validated on Western Blot.
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纯化方法
- Affinity Purified
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免疫原
- The immunogen is a synthetic peptide directed towards the middle region of Rat Ccdc42
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anti-Coiled-Coil Domain Containing 42 (CCDC42) (AA 221-312) antibody
CCDC42 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Coiled-Coil Domain Containing 42 (CCDC42) (AA 1-242) antibodyCCDC42 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Coiled-Coil Domain Containing 42 (CCDC42) (AA 161-210) antibodyCCDC42 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Coiled-Coil Domain Containing 42 (CCDC42) (AA 121-150) antibodyCCDC42 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB25993 unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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说明
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Antigen size: 316 AA
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限制
- 仅限研究用
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- by
- Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University of Göttingen
- No.
- #103398
- 日期
- 2018.10.12
- 抗原
- CCDC42
- Lot Number
- QC28887-41199
- Method validated
- Immunocytochemistry
- Positive Control
- NIH/3T3 cells transfected with Cddc42-mCherry expression vector
- Negative Control
- Non-transfected NIH/3T3 cells
- Notes
Passed. ABIN2785068 specifically detects Ccdc42-Cherry when exogenously expressed. Background staining is low.
- Primary Antibody
- ABIN2785068
- Secondary Antibody
- goat anti-rabbit 488 antibody (MoBiotec, MFP-A1008, lot 2806F058)
- Full Protocol
- Grow NIH/3T3 cells (ATCC, CRL-1658) in DMEM+GlutaMAX (Gibco, 10567-014, lot 1922818) supplemented with fetal bovine serum (Gibco, 10500-064, lot 08FO477K) and Pen/Strep (Gibco, 15140-122, lot 1924797) at 37°C and 5% CO2 to 70% confluency.
- Transfect cells with Ccdc42 tagged mCherry (Ccdc42-Cherry) expression vector using EndofectinMax (GeneCopoeia) following the manufacturer´s instructions.
- Grow cells for 24h.
- Fix cells in methanol for 10min at -20°C followed by incubation in 0.3% Triton X-100 for 10min at 4°C.
- Block cells in PBS containing 1% bovine serum albumin and 0.5% Tween-20 (PBT) for 1h at RT.
- Incubate cells with primary rabbit anti-Ccdc42 antibody (antibodies-online, ABIN2785068, QC28887-41199) diluted 1:100 in PBS for 1h at 37°C.
- Wash cells 3x 5min with 1x PBS.
- Incubate cells with secondary goat anti-rabbit 488 antibody (MoBiotec, MFP-A1008, lot 2806F058) diluted 1:300 for 1h at 37°C in PBS.
- DNA was counterstained with DAPI (4’, 6-Diamidino-2-phenylindole; Sigma, D-9542).
- Image acquisition on Zeiss LSM 510 confocal microscope and processing using Adobe Photoshop 5.0.
- Experimental Notes
生效 #103398 (Immunocytochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Cells were transfected with Ccdc42 tagged with mCherry (red) and incubated with ABIN2785068 (green). The pictures on the right show the red and green channels merged with DAPI counterstain (blue).]()
Cells were transfected with Ccdc42 tagged with mCherry (red) and incubated with ABIN2785068 (green). The pictures on the right show the red and green channels merged with DAPI counterstain (blue).
Full Methods -
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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: "Essential Role of CFAP53 in Sperm Flagellum Biogenesis." in: Frontiers in cell and developmental biology, Vol. 9, pp. 676910, (2021) (PubMed).
: "CCDC42 Localizes to Manchette, HTCA and Tail and Interacts With ODF1 and ODF2 in the Formation of the Male Germ Cell Cytoskeleton." in: Frontiers in cell and developmental biology, Vol. 7, pp. 151 (PubMed).
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: "Essential Role of CFAP53 in Sperm Flagellum Biogenesis." in: Frontiers in cell and developmental biology, Vol. 9, pp. 676910, (2021) (PubMed).
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- CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))
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别名
- Ccdc42
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背景
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The function of this protein remains unknown.
Alias Symbols: RGD1565925
Protein Size: 316 -
分子量
- 34 kDa
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基因ID
- 303234
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NCBI登录号
- NM_001107009, NP_001100479
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UniProt
- D3ZAZ8
抗原
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