DBT 抗体 (N-Term)
Quick Overview for DBT 抗体 (N-Term) (ABIN2784728)
抗原
See all DBT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- NYVCFFGYPS FKYSHPHHFL KTTAALRGQV VQFKLSDIGE GIREVTVKEW
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预测反应
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 83%, Zebrafish: 93%
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产品特性
- This is a rabbit polyclonal antibody against DBT. It was validated on Western Blot using a cell lysate as a positive control.
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纯化方法
- Affinity Purified
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免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of human DBT
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anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 35-84) antibody
DBT 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 猴, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 1-482) antibodyDBT 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 1-482) antibodyDBT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (N-Term) antibodyDBT 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) antibodyDBT 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 1G2 unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 140-259) antibodyDBT 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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说明
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Antigen size: 482 AA
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))
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别名
- DBT
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背景
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The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: BCATE2, E2, E2B, MGC9061
Protein Interaction Partner: HUWE1, UBC, PARK2, IFIT2, ABCC1, DLD, AGO4, AGO3, SRRM2, USP16, ADI1,
Protein Size: 482 -
分子量
- 46 kDa
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基因ID
- 1629
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NCBI登录号
- NM_001918, NP_001909
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UniProt
- P11182
抗原
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