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MED25 抗体 (N-Term)

Cited in 1 publication. This 兔 多克隆 anti-MED25 antibody specifically detects MED25 in WB 和 ChIP. The antibody is reactive with 人, 小鼠, 大鼠, Cow, 犬, Pig, 豚鼠 和 斑马鱼 samples.
产品编号 ABIN2784291
发货至: 中国
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Quick Overview for MED25 抗体 (N-Term) (ABIN2784291)

抗原

See all MED25 抗体
MED25 (Mediator Complex Subunit 25 (MED25))

适用

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人, 小鼠, 大鼠, Cow, 犬, Pig, 豚鼠, 斑马鱼

宿主

  • 21

克隆类型

  • 21
多克隆

标记

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This MED25 antibody is un-conjugated

应用范围

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Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)
  • 抗原表位

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    N-Term

    序列

    EGLRKHYLLP AIEYFNGGPP AETDFGGDYG GTQYSLVVFN TVDCAPESYV

    预测反应

    Cow: 100%, Dog: 100%, Guinea Pig: 85%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%

    产品特性

    This is a rabbit polyclonal antibody against MED25. It was validated on Western Blot.

    纯化方法

    Affinity Purified

    免疫原

    The immunogen is a synthetic peptide directed towards the N terminal region of human MED25
  • 应用备注

    Optimal working dilutions should be determined experimentally by the investigator.

    说明

    Antigen size: 747 AA

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze-thaw cycles.

    储存条件

    -20 °C

    储存方法

    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Sela, Conkright, Chen, Gilmore, Washburn, Florens, Conaway, Conaway: "Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6?." in: The Journal of biological chemistry, Vol. 288, Issue 36, pp. 26179-87, (2013) (PubMed).

  • 抗原

    MED25 (Mediator Complex Subunit 25 (MED25))

    别名

    MED25

    背景

    This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
    Alias Symbols: ACID1, ARC92, CMT2B2, DKFZp434K0512, MGC70671, P78, PTOV2, TCBAP0758
    Protein Interaction Partner: UBC, MED19, MED26, EPAS1, NOTCH1, DREB2A, CTDP1, MED6, THRA, RXRA, RARA, MED1, NR3C1, ESR1, CREBBP, SREBF1, MED25, MED15, MED16, MED13, MED12, MED7, MED17, MED23, MED14, CDK8, ZC3H13, QKI, TRIP4, TADA2A, MED28, OBFC1, TRRAP, UL48, MED9, MED8, HCFC1, MED10
    Protein Size: 747

    分子量

    78 kDa

    基因ID

    81857

    NCBI登录号

    NM_030973, NP_112235

    UniProt

    Q71SY5

    途径

    Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response
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