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RFT1 抗体 (N-Term)

This 兔 多克隆 antibody specifically detects RFT1 in WB. It exhibits reactivity toward 人, 犬, 小鼠, Cow, 豚鼠, 马, 兔 和 大鼠.
产品编号 ABIN311263
发货至: 中国
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Quick Overview for RFT1 抗体 (N-Term) (ABIN311263)

抗原

See all RFT1 抗体
RFT1 (RFT1 Homolog (RFT1))

适用

  • 14
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
人, 犬, 小鼠, Cow, 豚鼠, 马, 兔, 大鼠

宿主

  • 13
  • 1

克隆类型

  • 14
多克隆

标记

  • 9
  • 1
  • 1
  • 1
  • 1
  • 1
This RFT1 antibody is un-conjugated

应用范围

  • 13
  • 9
  • 8
  • 3
  • 1
Western Blotting (WB)
  • 抗原表位

    • 8
    • 3
    • 2
    • 1
    • 1
    • 1
    N-Term

    序列

    GTQRDWSQTL NLLWLTVPLG VFWSLFLGWI WLQLLEVPDP NVVPHYATGV

    预测反应

    Cow: 92%, Dog: 100%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 85%, Rabbit: 92%, Rat: 92%

    产品特性

    This is a rabbit polyclonal antibody against RFT1. It was validated on Western Blot using a cell lysate as a positive control.

    纯化方法

    Affinity Purified

    免疫原

    The immunogen is a synthetic peptide directed towards the N terminal region of human RFT1
  • 应用备注

    Optimal working dilutions should be determined experimentally by the investigator.

    说明

    Antigen size: 541 AA

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze-thaw cycles.

    储存条件

    -20 °C

    储存方法

    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • 抗原

    RFT1 (RFT1 Homolog (RFT1))

    别名

    RFT1

    背景

    N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a!!Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation.N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation (Helenius et al., 2002 [PubMed 11807558]).[supplied by OMIM].
    Alias Symbols: CDG1N
    Protein Interaction Partner: PSMA3, UBC, NEDD4,
    Protein Size: 541

    分子量

    60 kDa

    基因ID

    91869

    NCBI登录号

    NM_052859, NP_443091

    UniProt

    Q96AA3
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