RFT1 抗体 (N-Term)
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北京 101111
Quick Overview for RFT1 抗体 (N-Term) (ABIN2784093)
抗原
See all RFT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- RFT1 Antibody - N-terminal region
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序列
- GTQRDWSQTL NLLWLTVPLG VFWSLFLGWI WLQLLEVPDP NVVPHYATGV
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预测反应
- Cow: 92%, Dog: 100%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 85%, Rabbit: 92%, Rat: 92%
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产品特性
- This is a rabbit polyclonal antibody against RFT1. It was validated on Western Blot using a cell lysate as a positive control.
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纯化方法
- Affinity Purified
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免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of human RFT1
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anti-RFT1 Homolog (RFT1) (AA 513-541), (C-Term) antibody
RFT1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB27788 unconjugated
anti-RFT1 Homolog (RFT1) (AA 74-123) antibodyRFT1 适用: 人, 犬, 小鼠, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-RFT1 Homolog (RFT1) antibodyRFT1 适用: 人, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-RFT1 Homolog (RFT1) (AA 211-320) antibodyRFT1 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-RFT1 Homolog (RFT1) (AA 520-549), (C-Term) antibodyRFT1 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8110451
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- prevent freeze-thaw cycles
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储存条件
- 4 °C,-20 °C
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储存方法
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.
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- RFT1 (RFT1 Homolog (RFT1))
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别名
- RFT1
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背景
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Background Information: N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a ! !Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation.N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation (Helenius et al., 2002 [PubMed 11807558]).[supplied by OMIM].
Gene Name: RFT1 homolog (S. cerevisiae)
Alternative Symbols: CDG1N
Protein Name: Protein RFT1 homolog
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分子量
- 60kDa
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基因ID
- 91869
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NCBI登录号
- NP_443091
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UniProt
- Q96AA3
抗原
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