PDHA1 抗体 (N-Term)
Quick Overview for PDHA1 抗体 (N-Term) (ABIN2783235)
抗原
See all PDHA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- MRKMLAAVSR VLSGASQKPA SRVLVASRNF ANDATFEIKK CDLHRLEEGP
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预测反应
- Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
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产品特性
- This is a rabbit polyclonal antibody against PDHA1. It was validated on Western Blot using a cell lysate as a positive control.
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纯化方法
- Protein A purified
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免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of human PDHA1
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anti-Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) (AA 30-390) antibody
PDHA1 适用: 人 WB, IHC, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) (AA 1-390) antibodyPDHA1 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) (AA 30-390) antibodyPDHA1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) (C-Term) antibodyPDHA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) (pSer293) antibodyPDHA1 适用: 人 WB, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) (AA 223-390) antibodyPDHA1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) antibodyPDHA1 适用: 人, 小鼠 WB, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) (C-Term) antibodyPDHA1 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 斑马鱼, 豚鼠, 兔, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) (pSer293) antibodyPDHA1 适用: 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) (C-Term) antibodyPDHA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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说明
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Antigen size: 390 AA
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))
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别名
- PDHA1
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背景
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The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT, MIM 608770) (E2, EC 2.3.1.12), and dihydrolipoyl dehydrogenase (DLD, MIM 238331) (E3, EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: PDHA, PDHCE1A, PHE1A
Protein Interaction Partner: HUWE1, SUMO2, UBC, GINS4, DCPS, NCDN, VCL, PPP1R2, MSN, ASL, ADRB2, ITGA4, HNRNPDL, PDHX, SDHA, PDHB, OGDH, GTF2I, DLAT, CUL3, SIRT7, ATG101, WDR20, BABAM1, USP19, PDP1, EIF6, PDK4, PDK3, PDK2, PDK1, PDHA1, DGKE,
Protein Size: 390 -
分子量
- 40 kDa
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基因ID
- 5160
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NCBI登录号
- NM_000284, NP_000275
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UniProt
- P08559
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途径
- Warburg Effect
抗原
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