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VARS 抗体 (Middle Region)

Cited in 2+ publications. The 兔多克隆 anti-VARS antibody (ABIN2782517) specifically detects VARS in WB 和 IHC. The antibody is reactive with 人, 小鼠, 大鼠, Cow, 马, 兔, 犬, 豚鼠, 斑马鱼和 Saccharomyces cerevisiae samples.

WB Suggested Anti-VARS Antibody Titration: 1 ug/mlPositive Control: Wildtype Neurospora crassa (VARS 抗体 (Middle Region))

VARS antibody - middle region Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue Observed Staining: Cytoplasm of pneumocytes Primary Antibody Concentration: 1:100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0. (VARS 抗体 (Middle Region))

IHC

3 images

(2 references)

产品编号 ABIN2782517

发货至: 中国

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Quick Overview for VARS 抗体 (Middle Region) (ABIN2782517)

抗原

See all VARS 抗体

VARS (Valyl-tRNA Synthetase (VARS))

适用

人, 小鼠, 大鼠, Cow, 马, 兔, 犬, 豚鼠, 斑马鱼, Saccharomyces cerevisiae

宿主

兔

克隆类型

多克隆

标记

This VARS antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product VARS Primary Antibody

抗原表位
4

2

2

2

2

2

1

1

1

1

1
Middle Region

序列

VFDEFVDMDF GTGAVKITPA HDQNDYEVGQ RHGLEAISIM DSRGALINVP

预测反应

Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 86%, Zebrafish: 86%

产品特性

This is a rabbit polyclonal antibody against VARS. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the middle region of human VARS
anti-Valyl-tRNA Synthetase (VARS) (AA 1-280) antibody
VARS 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Valyl-tRNA Synthetase (VARS) (AA 186-421) antibody
VARS 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Valyl-tRNA Synthetase (VARS) antibody
VARS 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Valyl-tRNA Synthetase (VARS) (AA 1-280) antibody
VARS 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Valyl-tRNA Synthetase (VARS) (AA 576-625) antibody
VARS 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, 豚鼠, Pig, 斑马鱼, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Valyl-tRNA Synthetase (VARS) (AA 994-1102) antibody
VARS 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Valyl-tRNA Synthetase (VARS) (AA 994-1102) antibody
VARS 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B7 unconjugated

anti-Valyl-tRNA Synthetase (VARS) (C-Term) antibody
VARS 适用: 人, 小鼠, Cow, 马, 兔, 犬, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-Valyl-tRNA Synthetase (VARS) antibody
VARS 适用: 人 DB 宿主: 小鼠 Monoclonal VARSA7E6 unconjugated

anti-Valyl-tRNA Synthetase (VARS) (Middle Region) antibody
VARS 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 1264 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Duarte, Videira: "Defective valyl-tRNA synthetase hampers the mitochondrial respiratory chain in Neurospora crassa." in: The Biochemical journal, Vol. 448, Issue 3, pp. 297-306, (2012) (PubMed).

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).
抗原

VARS (Valyl-tRNA Synthetase (VARS))

别名

VARS

背景

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. VARS belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: G7A, VARS2, VARS1
Protein Interaction Partner: CALCOCO2, SUMO2, SART3, STAU1, UBC, EED, CCT4, KNTC1, AIMP1, CCT3, QARS, EEF1G, EEF1D, EEF1B2, FBXO6, YWHAQ, MAPK11, VCAM1, ITGA4, FN1, LARS, CAD, UBD, SHC1, CUL3, CDK2, CHD7, CACNA1A, ATG101, OTUD5, DMWD, EEF1A1, EEF1A2, TRAF6, TFE3, HLA-B, EPB41,
Protein Size: 1264

分子量

140 kDa

基因ID

7407

NCBI登录号

NM_006295, NP_006286

UniProt

P26640