CDC27 抗体 (Middle Region)
Quick Overview for CDC27 抗体 (Middle Region) (ABIN2781488)
抗原
See all CDC27 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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序列
- KLKMKFPPKI PNRKTKSKTN KGGITQPNIN DSLEITKLDS SIISEGKIST
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预测反应
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Rabbit: 100%, Rat: 100%
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产品特性
- This is a rabbit polyclonal antibody against CDC27. It was validated on Western Blot using a cell lysate as a positive control.
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纯化方法
- Affinity Purified
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免疫原
- The immunogen is a synthetic peptide directed towards the middle region of human CDC27
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anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 551-830) antibody
CDC27 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) antibodyCDC27 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 5C12 unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 258-431) antibodyCDC27 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 1-830) antibodyCDC27 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 395-444) antibodyCDC27 适用: 人, 大鼠, Cow, 马, 兔, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (Center) antibodyCDC27 适用: 人, Cow WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 814-823), (C-Term) antibodyCDC27 适用: 人, 大鼠, 小鼠, 犬, Cow, 非洲爪蟾 WB, IP, IHC (p), EIA 宿主: 小鼠 Monoclonal AF3-1 unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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说明
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Antigen size: 824 AA
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
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别名
- CDC27
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背景
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The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: APC3, CDC27Hs, D0S1430E, D17S978E, HNUC, NUC2, ANAPC3
Protein Interaction Partner: ANAPC2, UBE2S, PTTG1, FBXO43, MAD2L1, COMT, CDC20, CCNB1, BUB1B, UBC, BIRC6, ANAPC4, CDH1, FZR1, MIB1, HECW2, ANAPC11, PHF8, CUEDC2, Sirt2, Dlg4, GKN1, ANAPC7, ANAPC5, DCPS, ADNP, MDC1, PTEN, TRIM33, REV1, ANAPC15, VCP, MAD2L2, NOTCH1, PAXIP1, UBE4B, TTK,
Protein Size: 824 -
分子量
- 92 kDa
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基因ID
- 996
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NCBI登录号
- NM_001256, NP_001247
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UniProt
- P30260
抗原
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