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SMAD2 抗体 (N-Term)

Name: SMAD2 抗体 (N-Term)
SKU: ABIN2780721
Availability: OutOfStock

SMAD2 适用: 人, 小鼠, 大鼠, Cow, 兔, 斑马鱼, 绵羊, 犬, 马, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated

WB Suggested Anti-SMAD2 Antibody Titration: 2.

Lanes: Lane1: 2ug HEK293lysate Lane2: 2ug SMAD2 transfected HEK293 lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: SMAD2 Submitted by: Dominique Alfandari, University of Massachusetts

Host: Rat Target Name: SMAD2 Sample Tissue: Rat Liver Antibody Dilution: 1ug/ml

3 images

产品编号 ABIN2780721

发货至: 中国

Datasheet as PDF

抗原 See all SMAD2 抗体

SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
抗原表位
46

37

30

26

21

16

9

8

7

7

7

6

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
233

149

116

11

11

9

8

7

7

7

6

5

4

4

3

3

1

1
人, 小鼠, 大鼠, Cow, 兔, 斑马鱼, 绵羊, 犬, 马, 豚鼠
宿主
236

10
兔
Compatible Secondaries
克隆类型
212

34
多克隆
标记
124

18

12

10

8

8

8

8

8

7

7

5

5

5

5

5

3
This SMAD2 antibody is un-conjugated
应用范围
208

101

64

53

52

43

41

39

18

14

10

9

5

2

2

1

1
Western Blotting (WB)

序列

VVKRLLGWKK SAGGSGGAGG GEQNGQEEKW CEKAVKSLVK KLKKTGRLDE

预测反应

Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

产品特性

This is a rabbit polyclonal antibody against SMAD2. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Protein A purified

免疫原

The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD2

Top Product

Discover our top product SMAD2 Primary Antibody
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3G9 unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibody
SMAD2 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 5G7 unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 2-467) antibody
SMAD2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer423), (pSer425), (pSer465), (pSer467) antibody
SMAD2 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibody
KO Validated SMAD2 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (C-Term) antibody
SMAD2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D10 unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer467) antibody
SMAD2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pThr183), (pThr221) antibody
SMAD2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (N-Term) antibody
SMAD2 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 467 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
抗原

SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

别名

SMAD2 (SMAD2 产品)

别名

JV18 antibody, JV18-1 antibody, MADH2 antibody, MADR2 antibody, hMAD-2 antibody, hSMAD2 antibody, jv18 antibody, Xmad2 antibody, madh2 antibody, madr2 antibody, XSmad2 antibody, hmad-2 antibody, hsmad2 antibody, jv18-1 antibody, smad-2 antibody, fj43c06 antibody, wu:fj43c06 antibody, 7120426M23Rik antibody, Madh2 antibody, Madr2 antibody, Smad-2 antibody, mMad2 antibody, MAD homolog 2 antibody, Mothers against DPP homolog 2 antibody, SMAD 2 antibody, Smad2 antibody, SMAD family member 2 antibody, SMAD family member 2-Z antibody, SMAD family member 2 S homeolog antibody, SMAD2 antibody, SMAD2Z antibody, smad2 antibody, smad2.S antibody, Smad2 antibody

背景

SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
Alias Symbols: JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2
Protein Interaction Partner: HDAC2, UBC, SMAD4, SKIL, ANP32B, TUBA1B, SYT1, SOD1, ANP32E, PTMS, NEFM, JUN, ATP5I, RHOA, OTUB1, ZFYVE9, PPARG, TP53, TGFBR1, TRIM33, HCVgp1, TSC2, FHL3, FHL2, FHL1, CSNK1D, KLF5, SH2D2A, SKI, LCK, IRF7, IRF3, RNF111, LEMD3, PIK3CA, ST13, ST5, SRI, SPTBN
Protein Size: 467

分子量

51 kDa

基因ID

4087

NCBI登录号

NM_005901, NP_005892

UniProt

Q15796

途径

Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus