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FHL1 抗体 (C-Term)

Name: FHL1 抗体 (C-Term)
SKU: ABIN2775759
Availability: OutOfStock

This anti-FHL1 antibody is a 兔多克隆 antibody detecting FHL1 in WB 和 IHC. Suitable for 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 绵羊, 马和兔. This Primary Antibody has been cited in 12+ publications.

WB Suggested Anti-FHL1 Antibody Titration: 0. (FHL1 抗体 (C-Term))

IHC

Host: Mouse Target Name: FHL1 Sample Tissue: Mouse Skeletal Muscle Antibody Dilution: 1ug/ml (FHL1 抗体 (C-Term))

Host: Rabbit Target Name: FHL1 Sample Type: Human Fetal Heart Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1ug/ml Peptide Concentration: 5ug/ml Lysate Quantity: 25ug/lane/lane Gel Concentration: 0. (FHL1 抗体 (C-Term))

4 images

(12 references)

产品编号 ABIN2775759

发货至: 中国

Quick Overview for FHL1 抗体 (C-Term) (ABIN2775759)

抗原

See all FHL1 抗体

FHL1 (Four and A Half LIM Domains 1 (FHL1))

适用

人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 绵羊, 马, 兔

宿主

兔

克隆类型

多克隆

标记

This FHL1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product FHL1 Primary Antibody

抗原表位
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1
C-Term

序列

YYCVDCYKNF VAKKCAGCKN PITGFGKGSS VVAYEGQSWH DYCFHCKKCS

预测反应

Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%

产品特性

This is a rabbit polyclonal antibody against FHL1. It was validated on Western Blot and immunohistochemistry.

纯化方法

Protein A purified

免疫原

The immunogen is a synthetic peptide directed towards the C terminal region of human FHL1
anti-Four and A Half LIM Domains 1 (FHL1) (AA 23-120) antibody
FHL1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A9 unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) antibody
FHL1 适用: 人, 小鼠, 大鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) (Internal Region) antibody
Verified FHL1 适用: 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) antibody
FHL1 适用: 人, 大鼠, 犬 WB, IF, FACS 宿主: 小鼠 Monoclonal 2E11 unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) (AA 51-100) antibody
FHL1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 绵羊, 兔, Pig, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) (AA 214-240), (C-Term) antibody
FHL1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19645 unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) (N-Term) antibody
FHL1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 绵羊, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) (AA 13-240) antibody
FHL1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) (AA 1-280) antibody
FHL1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) (AA 1-280) antibody
FHL1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 280 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Le Thanh, Meinke, Korfali, Srsen, Robson, Wehnert, Schoser, Sewry, Schirmer: "Immunohistochemistry on a panel of Emery-Dreifuss muscular dystrophy samples reveals nuclear envelope proteins as inconsistent markers for pathology." in: Neuromuscular disorders : NMD, Vol. 27, Issue 4, pp. 338-351, (2017) (PubMed).

Xue, Schoser, Rao, Quadrelli, Vaglio, Rupp, Beichler, Nelson, Schapacher-Tilp, Windpassinger, Wilcox: "Exome Sequencing Identified a Splice Site Mutation in FHL1 that Causes Uruguay Syndrome, an X-Linked Disorder With Skeletal Muscle Hypertrophy and Premature Cardiac Death." in: Circulation. Cardiovascular genetics, Vol. 9, Issue 2, pp. 130-5, (2016) (PubMed).

Ma, Mao, Shen, Zheng, Li, Liu, Ni: "Atractylenolide I-mediated Notch pathway inhibition attenuates gastric cancer stem cell traits." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 353-9, (2014) (PubMed).

Fujii, Hayashi, Kawamura, Higuchi, Tsugawa, Ohyagi, Hayashi, Nishino, Kira: "A case of adult-onset reducing body myopathy presenting a novel clinical feature, asymmetrical involvement of the sternocleidomastoid and trapezius muscles." in: Journal of the neurological sciences, Vol. 343, Issue 1-2, pp. 206-10, (2014) (PubMed).

Koike, Kasamatsu, Iyoda, Saito, Kouzu, Koike, Sakamoto, Ogawara, Tanzawa, Uzawa: "High prevalence of epigenetic inactivation of the human four and a half LIM domains 1 gene in human oral cancer." in: International journal of oncology, Vol. 42, Issue 1, pp. 141-50, (2012) (PubMed).

Sharma, Shathasivam, Ignatchenko, Kislinger, Gramolini: "Identification of an FHL1 protein complex containing ACTN1, ACTN4, and PDLIM1 using affinity purifications and MS-based protein-protein interaction analysis." in: Molecular bioSystems, Vol. 7, Issue 4, pp. 1185-96, (2011) (PubMed).

Poparic, Schreibmayer, Schoser, Desoye, Gorischek, Miedl, Hochmeister, Binder, Quasthoff, Wagner, Windpassinger, Malle: "Four and a half LIM protein 1C (FHL1C): a binding partner for voltage-gated potassium channel K(v1.5)." in: PLoS ONE, Vol. 6, Issue 10, pp. e26524, (2011) (PubMed).

Schoser, Goebel, Janisch, Quasthoff, Rother, Bergmann, Müller-Felber, Windpassinger: "Consequences of mutations within the C terminus of the FHL1 gene." in: Neurology, Vol. 73, Issue 7, pp. 543-51, (2009) (PubMed).

Gueneau, Bertrand, Jais, Salih, Stojkovic, Wehnert, Hoeltzenbein, Spuler, Saitoh, Verschueren, Tranchant, Beuvin, Lacene, Romero, Heath, Zelenika, Voit, Eymard, Ben Yaou, Bonne: "Mutations of the FHL1 gene cause Emery-Dreifuss muscular dystrophy." in: American journal of human genetics, Vol. 85, Issue 3, pp. 338-53, (2009) (PubMed).

Windpassinger, Schoser, Straub, Hochmeister, Noor, Lohberger, Farra, Petek, Schwarzbraun, Ofner, Löscher, Wagner, Lochmüller, Vincent, Quasthoff: "An X-linked myopathy with postural muscle atrophy and generalized hypertrophy, termed XMPMA, is caused by mutations in FHL1." in: American journal of human genetics, Vol. 82, Issue 1, pp. 88-99, (2008) (PubMed).

Quinzii, Vu, Min, Tanji, Barral, Grewal, Kattah, Camaño, Otaegui, Kunimatsu, Blake, Wilhelmsen, Rowland, Hays, Bonilla, Hirano: "X-linked dominant scapuloperoneal myopathy is due to a mutation in the gene encoding four-and-a-half-LIM protein 1." in: American journal of human genetics, Vol. 82, Issue 1, pp. 208-13, (2008) (PubMed).

McGrath, Mitchell, Coghill, Robinson, Brown: "Skeletal muscle LIM protein 1 (SLIM1/FHL1) induces alpha 5 beta 1-integrin-dependent myocyte elongation." in: American journal of physiology. Cell physiology, Vol. 285, Issue 6, pp. C1513-26, (2003) (PubMed).
抗原

FHL1 (Four and A Half LIM Domains 1 (FHL1))

别名

FHL1

背景

LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may play an important role during the early stages of skeletal muscle differentiation, specifically in alpha5beta1-integrin-mediated signaling pathways.
Alias Symbols: KYOT, SLIM, FHL-1, FHL1A, FHL1B, FLH1A, SLIM1, XMPMA, SLIM-1, SLIMMER
Protein Interaction Partner: SUMO2, UBC, SRPK1, SMAD4, SMAD3, SMAD2, LMNA, CSNK1D, NRIP1, ESR1, HIVEP3, SMURF1, UBE2E2, STAT4, RING1, RBPJ, FHL1, KCNA5, PRNP, USP15, HHV8GK18_gp81, SP1, TXNIP, DEAF1, PDE4DIP, AKAP12, EED, HES1, DBN1, CBX4, FHL2, SRF, MYBPC1,
Protein Size: 280

分子量

32 kDa

基因ID

2273

NCBI登录号

NM_001449, NP_001440

UniProt

Q6IB30