我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

FHL1 抗体 (C-Term)

Cited in 12+ publications. The 兔多克隆 anti-FHL1 antibody (ABIN2775759) specifically detects FHL1 in WB 和 IHC. The antibody is reactive with 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 绵羊, 马和兔 samples.

IHC

Host: Mouse Target Name: FHL1 Sample Tissue: Mouse Skeletal Muscle Antibody Dilution: 1ug/ml (FHL1 抗体 (C-Term))

Host: Rabbit Target Name: FHL1 Sample Type: Human Fetal Heart Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1ug/ml Peptide Concentration: 5ug/ml Lysate Quantity: 25ug/lane/lane Gel Concentration: 0. (FHL1 抗体 (C-Term))

4 images

(12 references)

产品编号 ABIN2775759

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for FHL1 抗体 (C-Term) (ABIN2775759)

抗原

See all FHL1 抗体

FHL1 (Four and A Half LIM Domains 1 (FHL1))

适用

人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 绵羊, 马, 兔

宿主

兔

克隆类型

多克隆

标记

This FHL1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product FHL1 Primary Antibody

抗原表位
6

4

4

3

2

1

1

1

1

1

1

1

1

1

1
C-Term

序列

YYCVDCYKNF VAKKCAGCKN PITGFGKGSS VVAYEGQSWH DYCFHCKKCS

预测反应

Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%

产品特性

This is a rabbit polyclonal antibody against FHL1. It was validated on Western Blot and immunohistochemistry.

纯化方法

Protein A purified

免疫原

The immunogen is a synthetic peptide directed towards the C terminal region of human FHL1
anti-Four and A Half LIM Domains 1 (FHL1) (AA 23-120) antibody
FHL1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A9 unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) antibody
FHL1 适用: 人, 小鼠, 大鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) (Internal Region) antibody
Verified FHL1 适用: 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) antibody
FHL1 适用: 人, 大鼠, 犬 WB, IF, FACS 宿主: 小鼠 Monoclonal 2E11 unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) (AA 51-100) antibody
FHL1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 绵羊, 兔, Pig, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) antibody
KD Validated FHL1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB3885 unconjugated Recombinant Antibody

anti-Four and A Half LIM Domains 1 (FHL1) (AA 214-240), (C-Term) antibody
FHL1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19645 unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) (N-Term) antibody
FHL1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 绵羊, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) (AA 13-240) antibody
FHL1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Four and A Half LIM Domains 1 (FHL1) antibody
FHL1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 280 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Le Thanh, Meinke, Korfali, Srsen, Robson, Wehnert, Schoser, Sewry, Schirmer: "Immunohistochemistry on a panel of Emery-Dreifuss muscular dystrophy samples reveals nuclear envelope proteins as inconsistent markers for pathology." in: Neuromuscular disorders : NMD, Vol. 27, Issue 4, pp. 338-351, (2017) (PubMed).

Xue, Schoser, Rao, Quadrelli, Vaglio, Rupp, Beichler, Nelson, Schapacher-Tilp, Windpassinger, Wilcox: "Exome Sequencing Identified a Splice Site Mutation in FHL1 that Causes Uruguay Syndrome, an X-Linked Disorder With Skeletal Muscle Hypertrophy and Premature Cardiac Death." in: Circulation. Cardiovascular genetics, Vol. 9, Issue 2, pp. 130-5, (2016) (PubMed).

Ma, Mao, Shen, Zheng, Li, Liu, Ni: "Atractylenolide I-mediated Notch pathway inhibition attenuates gastric cancer stem cell traits." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 353-9, (2014) (PubMed).

Fujii, Hayashi, Kawamura, Higuchi, Tsugawa, Ohyagi, Hayashi, Nishino, Kira: "A case of adult-onset reducing body myopathy presenting a novel clinical feature, asymmetrical involvement of the sternocleidomastoid and trapezius muscles." in: Journal of the neurological sciences, Vol. 343, Issue 1-2, pp. 206-10, (2014) (PubMed).

Koike, Kasamatsu, Iyoda, Saito, Kouzu, Koike, Sakamoto, Ogawara, Tanzawa, Uzawa: "High prevalence of epigenetic inactivation of the human four and a half LIM domains 1 gene in human oral cancer." in: International journal of oncology, Vol. 42, Issue 1, pp. 141-50, (2012) (PubMed).

Sharma, Shathasivam, Ignatchenko, Kislinger, Gramolini: "Identification of an FHL1 protein complex containing ACTN1, ACTN4, and PDLIM1 using affinity purifications and MS-based protein-protein interaction analysis." in: Molecular bioSystems, Vol. 7, Issue 4, pp. 1185-96, (2011) (PubMed).

Poparic, Schreibmayer, Schoser, Desoye, Gorischek, Miedl, Hochmeister, Binder, Quasthoff, Wagner, Windpassinger, Malle: "Four and a half LIM protein 1C (FHL1C): a binding partner for voltage-gated potassium channel K(v1.5)." in: PLoS ONE, Vol. 6, Issue 10, pp. e26524, (2011) (PubMed).

Schoser, Goebel, Janisch, Quasthoff, Rother, Bergmann, Müller-Felber, Windpassinger: "Consequences of mutations within the C terminus of the FHL1 gene." in: Neurology, Vol. 73, Issue 7, pp. 543-51, (2009) (PubMed).

Gueneau, Bertrand, Jais, Salih, Stojkovic, Wehnert, Hoeltzenbein, Spuler, Saitoh, Verschueren, Tranchant, Beuvin, Lacene, Romero, Heath, Zelenika, Voit, Eymard, Ben Yaou, Bonne: "Mutations of the FHL1 gene cause Emery-Dreifuss muscular dystrophy." in: American journal of human genetics, Vol. 85, Issue 3, pp. 338-53, (2009) (PubMed).

Windpassinger, Schoser, Straub, Hochmeister, Noor, Lohberger, Farra, Petek, Schwarzbraun, Ofner, Löscher, Wagner, Lochmüller, Vincent, Quasthoff: "An X-linked myopathy with postural muscle atrophy and generalized hypertrophy, termed XMPMA, is caused by mutations in FHL1." in: American journal of human genetics, Vol. 82, Issue 1, pp. 88-99, (2008) (PubMed).

Quinzii, Vu, Min, Tanji, Barral, Grewal, Kattah, Camaño, Otaegui, Kunimatsu, Blake, Wilhelmsen, Rowland, Hays, Bonilla, Hirano: "X-linked dominant scapuloperoneal myopathy is due to a mutation in the gene encoding four-and-a-half-LIM protein 1." in: American journal of human genetics, Vol. 82, Issue 1, pp. 208-13, (2008) (PubMed).

McGrath, Mitchell, Coghill, Robinson, Brown: "Skeletal muscle LIM protein 1 (SLIM1/FHL1) induces alpha 5 beta 1-integrin-dependent myocyte elongation." in: American journal of physiology. Cell physiology, Vol. 285, Issue 6, pp. C1513-26, (2003) (PubMed).
抗原

FHL1 (Four and A Half LIM Domains 1 (FHL1))

别名

FHL1

背景

LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may play an important role during the early stages of skeletal muscle differentiation, specifically in alpha5beta1-integrin-mediated signaling pathways.
Alias Symbols: KYOT, SLIM, FHL-1, FHL1A, FHL1B, FLH1A, SLIM1, XMPMA, SLIM-1, SLIMMER
Protein Interaction Partner: SUMO2, UBC, SRPK1, SMAD4, SMAD3, SMAD2, LMNA, CSNK1D, NRIP1, ESR1, HIVEP3, SMURF1, UBE2E2, STAT4, RING1, RBPJ, FHL1, KCNA5, PRNP, USP15, HHV8GK18_gp81, SP1, TXNIP, DEAF1, PDE4DIP, AKAP12, EED, HES1, DBN1, CBX4, FHL2, SRF, MYBPC1,
Protein Size: 280

分子量

32 kDa

基因ID

2273

NCBI登录号

NM_001449, NP_001440

UniProt

Q6IB30