SRF 抗体 (N-Term)
Quick Overview for SRF 抗体 (N-Term) (ABIN2774664)
抗原
See all SRF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- ATGGYGPVSG AVSGAKPGKK TRGRVKIKME FIDNKLRRYT TFSKRKTGIM
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交叉反应
- 牛, 犬, 小鸡, 人, 小鼠, 大鼠
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预测反应
- Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%
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产品特性
- This is a rabbit polyclonal antibody against SRF. It was validated on Western Blot using a cell lysate as a positive control.
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纯化方法
- Protein A purified
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免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of human SRF
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anti-Serum Response Factor (SRF) (AA 101-250) antibody
SRF 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (AA 406-508) antibodySRF 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E1 unconjugated
anti-Serum Response Factor (SRF) (pSer99) antibodySRF 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (AA 406-508) antibodySRF 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Serum Response Factor (SRF) (N-Term) antibodySRF 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (AA 57-269) antibodySRF 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (AA 616-875) antibodySRF 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (pSer77) antibodySRF 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (AA 231-474) antibodySRF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB Suggested Anti-SRF Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator. -
说明
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Antigen size: 508 AA
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- SRF (Serum Response Factor (SRF))
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背景
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SRF is a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).
Alias Symbols: MCM1
Protein Interaction Partner: Camk2d, ZIC3, ELK1, SRFBP1, SRF, NFKB1, PRKDC, CIRBP, ALDH3A1, SUMO1, UBE2I, SHBG, TRIM63, KDM6A, MYOCD, MKL1, CNTN2, CSNK2A1, SMARCA4, PELP1, KAT5, HDAC4, EPC1, SMAD7, HDAC3, MKL2, ATF6, BARX2, MAPKAPK2, FHL3, GATA4, CAMK2A, HOPX, PYCARD, NKX2-3, MINPP1,
Protein Size: 508 -
分子量
- 52 kDa
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基因ID
- 6722
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NCBI登录号
- NM_003131, NP_003122
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UniProt
- P11831
抗原
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