BCKDHA 抗体 (N-Term)
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北京 101111
Quick Overview for BCKDHA 抗体 (N-Term) (ABIN2774132)
抗原
See all BCKDHA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- NVISGIPIYR VMDRQGQIIN PSEDPHLPKE KVLKLYKSMT LLNTMDRILY
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预测反应
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 93%
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产品特性
- This is a rabbit polyclonal antibody against BCKDHA. It was validated on Western Blot using a cell lysate as a positive control.
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纯化方法
- Affinity Purified
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免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of human BCKDHA
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anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 268-407) antibody
BCKDHA 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 347-445) antibodyBCKDHA 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 362-390), (C-Term) antibodyBCKDHA 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB20924 unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 276-445) antibodyBCKDHA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 1-445) antibodyBCKDHA 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 71-120) antibodyBCKDHA 适用: 人, 小鼠, 大鼠, 豚鼠, 兔, Cow, 马, 绵羊, 猴, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (N-Term) antibodyBCKDHA 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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说明
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Antigen size: 445 AA
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))
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别名
- BCKDHA
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背景
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The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD, EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998 [PubMed 9582350]). The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2, MIM 248610), and a homodimeric dihydrolipoamide dehydrogenase (E3, MIM 238331). E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene (MIM 248611) encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-675 BI910860.1 12-686 676-1196 BG742673.1 80-600 1197-1731 BM702667.1 48-582 1732-1763 BE223026.1 1-32 c 1764-1781 BQ018849.1 1-18 c
Alias Symbols: FLJ45695, MSU, MSUD1, OVD1A, BCKDE1A
Protein Interaction Partner: UBC, CUL3, BCKDHB, BCKDK,
Protein Size: 445 -
分子量
- 50 kDa
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基因ID
- 593
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NCBI登录号
- NM_000709, NP_000700
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UniProt
- P12694
抗原
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