Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
纯化方法
GroEL antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
免疫原
Anti-human GroEL mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GroEL amino acids 1-573 purified from E. coli.
GroEL
适用: 人
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Cy5.5
应用备注
GroEL antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000 ~ 1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.