HNK1, GLCATP, NK-1, HNK-1, LEU7, NK1, 151290, B3GAT1, Q9P2W7, GlcUAT-P, CD57, GlcUATP, GlcAT-P, 27087, NK 1, HNK 1, GlcAT P, GlcUAT P,Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo- orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl- sphingomyelin was the most effective, followed by palmitoyl- sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group (By similarity).
纯化方法
Protein A/G Chromatography
免疫原
Hybridoma produced from mice immunized with human peripheral blood mononuclear cells.
Antibody can be used for Western blotting (1-10 μg/mL), Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Immunofluorescence (2-20 μg/mL), Immunohistochemistry (2-20 μg/mL). Optimal concentration should be evaluated by serial dilutions.
限制
仅限研究用
缓冲液
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles