FeSOD 抗体
This anti-FeSOD antibody is a 兔 多克隆 antibody detecting FeSOD in WB. Suitable for Arabidopsis thaliana, Chlamydomonas reinhardtii, Oryza sativa 和 Zea mays. This Primary Antibody has been cited in 2+ publications.
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(2 references)Quick Overview for FeSOD 抗体 (ABIN2749232)
抗原
FeSOD
(Chloroplastic Fe-Dependent Superoxide Dismutase (FeSOD))
适用
Arabidopsis thaliana, Chlamydomonas reinhardtii, Oryza sativa, Zea mays
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB)
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交叉反应 （详细）
- No cross-reactivity with: cyanobacteria
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产品特性
- Expected / apparent Molecular Weight of the Antigene: 25 / 22 kDa
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纯化方法
- serum
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免疫原
- overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece
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应用备注
- Recommended Dilution: 1 : 1500 to 1 : 5000 with alkaline phosphatase (WB).
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说明
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The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 µM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 µL of sterile water.
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注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
储存条件
- -20 °C
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: "Fe sparing and Fe recycling contribute to increased superoxide dismutase capacity in iron-starved Chlamydomonas reinhardtii." in: The Plant cell, Vol. 24, Issue 6, pp. 2649-65, (2012) (PubMed).
: "Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts." in: The Plant cell, Vol. 17, Issue 4, pp. 1233-51, (2005) (PubMed).
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: "Fe sparing and Fe recycling contribute to increased superoxide dismutase capacity in iron-starved Chlamydomonas reinhardtii." in: The Plant cell, Vol. 24, Issue 6, pp. 2649-65, (2012) (PubMed).
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- FeSOD (Chloroplastic Fe-Dependent Superoxide Dismutase (FeSOD))
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背景
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AGI Code: At4g25100
Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD -
分子量
- expected: 25 kDa, apparent: 22 kDa
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UniProt
- A8IGH1
抗原
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