Cytokeratins 抗体 (Biotin)
(4 references)-
- 抗原
- Cytokeratins
- 适用
- 哺乳动物
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- Biotin
- 应用范围
- Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)
- 原理
- Anti-Cytokeratins Biotin
- 特异性
- The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.
- 交叉反应 （详细）
- Mammalian
- 纯化方法
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
- 免疫原
- Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
- 克隆位点
- C-11
- 亚型
- IgG1
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anti-Cytokeratins antibody
适用: 哺乳动物 ICC, IHC (p), IP, ICFC, WB 宿主: 小鼠 Monoclonal C-11 unconjugated
anti-Cytokeratins antibody (FITC)适用: 哺乳动物 ICFC 宿主: 小鼠 Monoclonal C-11 FITC
anti-Cytokeratins antibody (APC)适用: 哺乳动物 ICFC 宿主: 小鼠 Monoclonal C-11 APC
anti-Cytokeratins antibody (PE)适用: 哺乳动物 ICFC 宿主: 小鼠 Monoclonal C-11 PE
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- 应用备注
- Flow cytometry: Recommended dilution: 1 μg/mL. Intracellular staining.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats." in: Kidney international, Vol. 68, Issue 6, pp. 2572-81, (2005) (PubMed).
: "Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis." in: Research communications in molecular pathology and pharmacology, Vol. 101, Issue 2, pp. 115-26, (1999) (PubMed).
: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).
: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).
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: "Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats." in: Kidney international, Vol. 68, Issue 6, pp. 2572-81, (2005) (PubMed).
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- 抗原
- Cytokeratins
- 背景
- Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,cytokeratin, CYK, CK, KRT
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