(5 references)-
- 抗原 See all CD90.2 / Thy-1.2 products
- CD90.2 / Thy-1.2
- 适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD90.2 / Thy-1.2 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 特异性
- The mouse monoclonal antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90), it does not cross-react with Thy-1.1 alloantigen. Thy-1 is an 18-35 kDa cell surface GPI anchored glycoprotein (immunoglobulin superfamily), in mouse it is abundant on thymocytes and peripheral T lymphocytes.
- 无交叉反应
- 人, 大鼠
- 交叉反应 （详细）
- Mouse
- 纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- thymocytes from C3H/Di mice (Thy-1.2 positive)
- 克隆位点
- 1aG4-C5
- 亚型
- IgG3
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anti-CD90.2 / Thy-1.2 antibody (PE)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 PE
anti-CD90.2 / Thy-1.2 antibody (violetFluor™ 450)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 violetFluor™ 450
anti-CD90.2 / Thy-1.2 antibody (PE-Cy7)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 PE-Cy7
anti-CD90.2 / Thy-1.2 antibody适用: 小鼠 CyTox 宿主: 小鼠 Monoclonal unconjugated
anti-CD90.2 / Thy-1.2 antibody适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 unconjugated
anti-CD90.2 / Thy-1.2 antibody (Biotin)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 Biotin
anti-CD90.2 / Thy-1.2 antibody (FITC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 FITC
anti-CD90.2 / Thy-1.2 antibody (APC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 APC
anti-CD90.2 / Thy-1.2 antibody (SPRD)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 30-H12 SPRD
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- 应用备注
- Flow cytometry: Recommended dilution: 1-2 μg/mL.
- 说明
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Roles and regulation of Thy-1, a context-dependent modulator of cell phenotype." in: BioFactors (Oxford, England), Vol. 35, Issue 3, pp. 258-65, (2009) (PubMed).
: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, (2006) (PubMed).
: "Thy-1 glycoprotein and src-like protein-tyrosine kinase p53/p56lyn are associated in large detergent-resistant complexes in rat basophilic leukemia cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3611-5, (1993) (PubMed).
: "Monoclonal IgG3 (kappa) antibodies against murine Thy-1.2 antigen produced by murine hybridomas. Differences in the specificity of the antigen binding site and in the structure of the hinge region." in: Folia biologica, Vol. 28, Issue 6, pp. 377-94, (1983) (PubMed).
: "Establishment and characterization of permanent murine hybridomas secreting monoclonal anti-thy-1 antibodies." in: Journal of immunogenetics, Vol. 7, Issue 6, pp. 455-74, (1981) (PubMed).
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: "Roles and regulation of Thy-1, a context-dependent modulator of cell phenotype." in: BioFactors (Oxford, England), Vol. 35, Issue 3, pp. 258-65, (2009) (PubMed).
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- 抗原
- CD90.2 / Thy-1.2
- Abstract
- CD90.2 / Thy-1.2 产品
- 别名
- CD90 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, thymus cell antigen 1, theta antibody, Thy1 antibody
- 背景
- Thy-1.2 cell surface antigen,CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. In the mouse, CD90 is expressed mainly on thymocytes and peripheral T lymphocytes. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.,Thy-1.2
- 基因ID
- 21838
- UniProt
- P01831
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