IKZF2 抗体 (AA 51-107)
(4 references)Quick Overview for IKZF2 抗体 (AA 51-107) (ABIN2749084)
抗原
See all IKZF2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 51-107
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原理
- Anti-Helios Purified
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特异性
- The Armenian hamster monoclonal antibody 22F6 recognizes Helios, a transcription factor (intracellular antigen) expressed in some hematopoietic stem cells, and at high levels in thymic-derived regulatory T cells. The epitope is located between amino acids 51 and 107.
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交叉反应 （详细）
- Human, Mouse
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Peptide coresponding to the amino acids 51-107 of Helios
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亚型
- IgG
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anti-IKAROS Family Zinc Finger 2 (IKZF2) (AA 1-500) antibody
IKZF2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-IKAROS Family Zinc Finger 2 (IKZF2) (Internal Region) antibodyIKZF2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-IKAROS Family Zinc Finger 2 (IKZF2) (AA 396-445) antibodyIKZF2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-IKAROS Family Zinc Finger 2 (IKZF2) (AA 263-504) antibodyIKZF2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-IKAROS Family Zinc Finger 2 (IKZF2) (AA 51-107) antibody (PE)IKZF2 适用: 人, 小鼠 ICFC 宿主: 仓鼠 Monoclonal 22F6 PE
anti-IKAROS Family Zinc Finger 2 (IKZF2) (AA 21-120) antibodyIKZF2 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-IKAROS Family Zinc Finger 2 (IKZF2) (AA 25-75) antibodyIKZF2 适用: 人, 犬, 马, Cow WB, IHC, IHC (p), IP 宿主: 兔 Polyclonal unconjugated
anti-IKAROS Family Zinc Finger 2 (IKZF2) (AA 395-444) antibodyIKZF2 适用: 人, 犬, 马, 豚鼠, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-IKAROS Family Zinc Finger 2 (IKZF2) (AA 300-440) antibodyIKZF2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-IKAROS Family Zinc Finger 2 (IKZF2) (Middle Region) antibodyIKZF2 适用: 人, 小鼠, 大鼠, 犬, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Flow cytometry: Recommended dilution: 1-4 μg/mL.,Recommended protocol: 1) Perform staining of cell surface markers (CD25, CD4 etc.) for 20 min. at room temperature in the dark, 100 μL of peripheral blood. 2) Add 3 mL of PBS with 1 % BSA, centrifugate at 300g and discard the supernatant. Further steps perform on ice and with ice-cold reagents. 3) Resuspend the cells in 5 mL of cold fixation solution (Miltenyi Biotec) and incubate for 30 min. on ice. 4) Centrifugate for 5 min. at 1000 g, 4 °C, and discard the supernatant. 5) Resuspend the cells in 5 mL of ice-cold PBS with 1 % BSA. 6) Centrifugate for 5 min. at 1000 g, 4 °C, and discard the supernatant. 7) Resuspend the cells in 5 mL of ice-cold permeabilization solution (Miltenyi Biotec) and incubate 5 min. 8) Centrifugate for 5 min. at 1000 g, 4 °C, and discard the supernatant. 9) Resuspend the cells in μl of ice-cold permeabilization solution and add 20 μL of FcR blocking solution and incubate for 5 min. (4 °C, in the dark). 10) Perform intracellular staining of Helios for 30 min. (4 °C, in the dark) with 10 μL of anti-Helios PE antibody (22F6) in 100 μL. 11) Add 2 mL of ice-cold permeabilization solution and incubate for 5 min. at 4 °C in the dark. 12) Centrifugate for 5 min. at 1000 g, 4 °C, and discard the supernatant. 13) Resuspend the cells in 3 mL of ice-cold PBS with 1 % BSA. 14) Centrifugate for 5 min. at 1000 g, 4 °C, and discard the supernatant. 15) Resuspend the cells in 150 μL of ice-cold PBS with 1 % BSA and measure on a flow cytometry device with appropriate setting as soon as possible. Keep cold until measuring.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Foxp3? regulatory T cells delay expulsion of intestinal nematodes by suppression of IL-9-driven mast cell activation in BALB/c but not in C57BL/6 mice." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003913, (2014) (PubMed).
: "Donor lymphocyte count and thymic activity predict lymphocyte recovery and outcomes after matched-sibling hematopoietic stem cell transplant." in: Haematologica, Vol. 98, Issue 3, pp. 346-52, (2013) (PubMed).
: "Helios is associated with CD4 T cells differentiating to T helper 2 and follicular helper T cells in vivo independently of Foxp3 expression." in: PLoS ONE, Vol. 6, Issue 6, pp. e20731, (2011) (PubMed).
: "Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog." in: Immunology, Vol. 132, Issue 1, pp. 111-22, (2010) (PubMed).
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: "Foxp3? regulatory T cells delay expulsion of intestinal nematodes by suppression of IL-9-driven mast cell activation in BALB/c but not in C57BL/6 mice." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003913, (2014) (PubMed).
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- IKZF2 (IKAROS Family Zinc Finger 2 (IKZF2))
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别名
- Helios
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背景
- IKAROS family zinc finger 2,Helios, also known as IKZF2 (Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells.,ANF1A2, IKZF2
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基因ID
- 22807
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UniProt
- Q9UKS7
抗原
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