CD247 抗体 (pTyr72)
(1 reference)Quick Overview for CD247 抗体 (pTyr72) (ABIN2749060)
抗原
See all CD247 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pTyr72
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原理
- Anti-Hu CD3 zeta (pY72) Purified
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特异性
- The mouse monoclonal antibody EM-26 recognizes phosphorylated intracellular tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
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交叉反应 （详细）
- Human, Mouse
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH
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亚型
- IgG2b
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anti-CD247 Molecule (CD247) (AA 1-164) antibody
CD247 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) (AA 1-164) antibodyCD247 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 4A12-F6 unconjugated
anti-CD247 Molecule (CD247) (AA 88-153) antibodyCD247 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) (AA 50-164) antibodyCD247 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) antibodyCD247 适用: 人 ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 4B10 unconjugated
anti-CD247 Molecule (CD247) antibodyCD247 适用: 人 WB, IHC, FACS 宿主: 兔 Monoclonal unconjugated
anti-CD247 Molecule (CD247) (AA 1-165) antibodyCD247 适用: 人 WB, IP, IHC (p), EIA 宿主: 小鼠 Monoclonal 4A12-F6 unconjugated
anti-CD247 Molecule (CD247) (AA 51-100) antibodyCD247 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) (C-Term) antibodyCD247 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) (AA 1-165) antibodyCD247 适用: 人 WB, ELISA, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 4A12-F6 unconjugated
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应用备注
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Western blotting: Recommended dilution: 2 - 5 μg/mL, positive control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, non-reducing conditions recommended.
Flow cytometry: Intracellular staining, recommended dilution: 1-9 μg/mL, positive control: Jurkat cells treated with pervanadate, T-cells from lymph nodes of OT-1 mouse treated with pervanadate. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR-CD3 signalling." in: Immunology letters, Vol. 130, Issue 1-2, pp. 43-50, (2010) (PubMed).
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: "Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR-CD3 signalling." in: Immunology letters, Vol. 130, Issue 1-2, pp. 43-50, (2010) (PubMed).
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- CD247 (CD247 Molecule (CD247))
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别名
- CD3 zeta
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背景
- CD247 Molecule,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 zeta chain, CD247, T3Z, TCRz, IMD25
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基因ID
- 919
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UniProt
- P20963
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途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
抗原
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