Myc Tag 抗体 (C-Term) (Biotin)
(8 references)Quick Overview for Myc Tag 抗体 (C-Term) (Biotin) (ABIN2749043)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- C-Term
-
原理
- Anti-c-Myc tag Biotin
-
特异性
- The antibody 9E10 can be used to detect the c-Myc tag.
-
交叉反应 （详细）
- Human, Recognizes fusion proteins in all species
-
纯化方法
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
-
免疫原
- Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
-
亚型
- IgG1
-
-
anti-Myc Tag (AA 408-439) antibody
Animal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: 兔 Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag (AA 408-439) antibodyAnimal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: 小鼠 Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag antibodyWB, IP, ELISA, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Myc Tag (AA 408-439) antibodyAnimal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: Human Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag (C-Term) antibody适用: 人 WB, IP, ELISA, IHC (fro), IHC (p), ICC, FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 unconjugated
anti-Myc Tag (C-Term) antibody (FITC)适用: 人 FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 FITC
anti-Myc Tag antibodyWB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 2G8D5 unconjugated
anti-Myc Tag (AA 408-439) antibodyWB, ELISA 宿主: 小鼠 Monoclonal 9E10- unconjugated
anti-Myc Tag (AA 410-419) antibodyWB 宿主: 山羊 Polyclonal unconjugated
anti-Myc Tag (AA 408-439), (C-Term) antibodyIF, IHC, FACS, StM 宿主: 小鼠 Monoclonal 9E10-3 unconjugated
-
-
应用备注
- Flow cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-5 μg/mL.
-
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store at 2-8°C. Do not freeze.
-
-
-
: "Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway." in: Biochemical pharmacology, Vol. 92, Issue 3, pp. 415-24, (2014) (PubMed).
: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." in: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, (2008) (PubMed).
: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." in: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, (2004) (PubMed).
: "Identification of epitope-like consensus motifs using mRNA display." in: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, (2002) (PubMed).
: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." in: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, (2002) (PubMed).
: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).
: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." in: Protein engineering, Vol. 14, Issue 10, pp. 803-6, (2001) (PubMed).
: "Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC." in: International journal of oncology, Vol. 13, Issue 6, pp. 1259-62, (1999) (PubMed).
-
: "Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway." in: Biochemical pharmacology, Vol. 92, Issue 3, pp. 415-24, (2014) (PubMed).
-
- Myc Tag
-
别名
- c-Myc tag
-
物质类
- Tag
-
背景
- MYC proto-oncogene,The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.,bHLH, MRTL, MYCC
-
基因ID
- 4609
-
UniProt
- P01106
抗原
-
