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TCR Vbeta5.3-Related 抗体

Cited in 1 publication. This anti-TCR Vbeta5.3-Related antibody is a 小鼠 单克隆 antibody detecting TCR Vbeta5.3-Related in FACS, IP, WB 和 Func. Suitable for 人.
产品编号 ABIN2749039
发货至: 中国
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Quick Overview for TCR Vbeta5.3-Related 抗体 (ABIN2749039)

抗原

TCR Vbeta5.3-Related

适用

宿主

  • 2
小鼠

克隆类型

  • 2
单克隆

标记

  • 2
非结合性

应用范围

Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)

克隆位点

MEM-262
  • 原理

    Anti-Hu TCR Vbeta5.3-related Purified Low Endotoxin

    特异性

    The antibody MEM-262 recognizes an extracellular epitope on beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

    交叉反应 (详细)

    Other not tested, Human

    纯化方法

    Purified by protein-A affinity chromatography.

    纯度

    > 95 % (by SDS-PAGE)

    内毒素水平

    Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

    免疫原

    Human thymoma cell line HPB-ALL.

    亚型

    IgG2a
  • 应用备注

    Western blotting: This antibody uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.
    Functional application: This antibody activates T cells (V beta 5-related subset).
    Flow cytometry: Recommended dilution: 1-4 μg/mL

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.4

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Do not freeze.
  • Pavlistová, Drbal, Hilgert, Horejsí: "A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor." in: Immunology letters, Vol. 88, Issue 2, pp. 105-8, (2003) (PubMed).

  • 抗原

    TCR Vbeta5.3-Related

    背景

    TCRVB5.3-related
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