Profilin 抗体 (full length)
This anti-Profilin antibody is a 兔 多克隆 antibody detecting Profilin in WB, ICC 和 IHC. Suitable for 人, Cow, 小鼠, Pig 和 大鼠.
Quick Overview for Profilin 抗体 (full length) (ABIN2745561)
抗原
See all Profilin (PFN) 抗体
Profilin (PFN)
适用
人, Cow, 小鼠, Pig, 大鼠
宿主
兔
克隆类型
多克隆
标记
This Profilin antibody is un-conjugated
应用范围
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
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抗原表位
- full length
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特异性
- Recognizes human, mouse, rat, pig, bovine and marsupial profilin.
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交叉反应
- 牛, 人, 小鼠, 猪, 大鼠
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纯化方法
- Antigen affinity purified.
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组件
- Contains Positive Control: Human platelet protein (Prod. No. AG-35T-0001), 500μg supplied at 5mg/ml in SDS sample buffer. Use 5μl (25μg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.
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免疫原
- Human full-length profilin.
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anti-Profilin (PFN) (AA 1-133) antibody
PFN 适用: Mercenaria (Clam) ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Profilin (PFN) antibodyPFN 适用: 人, Cow, 豚鼠, 兔 WB, ICC, IHC 宿主: 小鼠 Monoclonal 2H11 unconjugated
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应用备注
- Use 5 μL (25 μg) per lane for Western blotting of tricine gels (13 % acrylamide) or 15 % Laemmli gels.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- In PBS containing 1 mg/mL BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C. -
有效期
- 12 months
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- Profilin (PFN)
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别名
- Profilin
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背景
- Profilin (PFN1) is a ubiquitous small (12-15 kDa) phosphoinositide and poly-L-proline binding protein that plays a role in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes. Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma. Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth. Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion and possibly also in linking the actin cytoskeleton and endocytosis. Profilin has been found to associate with defined complexes containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK-II and HEM2/NAP1. Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18).
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UniProt
- P07737
抗原
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