(4 references)-
- 抗原
- Polyglutamate Chain
- 适用
- 所有种类
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Immunocytochemistry (ICC), Western Blotting (WB)
- 特异性
- Recognizes C-terminally located linear glutamate chains of 4 and more glutamate residues.
- 交叉反应
- 所有种类
- 免疫原
- Polyglutamate peptide.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- In PBS containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C. - 有效期
- 12 months
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: "Light microscopy of proteins in their ultrastructural context." in: Nature communications, Vol. 11, Issue 1, pp. 3850, (2020) (PubMed).
: "Essential function of the alveolin network in the subpellicular microtubules and conoid assembly in Toxoplasma gondii." in: eLife, Vol. 9, (2020) (PubMed).
: "A helical inner scaffold provides a structural basis for centriole cohesion." in: Science advances, Vol. 6, Issue 7, pp. eaaz4137, (2020) (PubMed).
: "Imaging cellular ultrastructures using expansion microscopy (U-ExM)." in: Nature methods, Vol. 16, Issue 1, pp. 71-74, (2019) (PubMed).
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: "Light microscopy of proteins in their ultrastructural context." in: Nature communications, Vol. 11, Issue 1, pp. 3850, (2020) (PubMed).
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- 抗原
- Polyglutamate Chain
- 背景
- Microtubules are key elements of the eukaryotic cytoskeleton that dynamically assemble from heterodimers of alpha- and beta-tubulin. Two different mechanisms can generate microtubule diversity: the expression of different alpha- and beta-tubulin genes, referred to as tubulin isotypes, and the generation of posttranslational modifications (PTMs) on alpha- and beta-tubulin. Tubulin PTMs include the well-known acetylation or phosphorylation, and others that have so far mostly been found on tubulin, detyrosination/tyrosination, polyglutamylation and polyglycylation. These PTMs might have evolved to specifically regulate tubulin and microtubule functions. Polyglutamylation is a PTM that occurs when secondary glutamate side chains are formed on gamma-carboxyl groups of glutamate residues in a protein. Enzymes catalyzing polyglutamylation belong to the TTL-like (TTLL, Tubulin tyrosine ligase-like) family of glutamylases. Deglutamylases, the enzymes that reverse polyglutamylation, were identified within a novel family of CCPs (cytosolic carboxypeptidase). Subtle differences in polyglutamylation can be seen on diverse microtubules in different cell types. The functions of these modifications remain to be studied. However, its wide distribution strengthens the idea that it could be involved in fine-tuning a range of microtubule functions.
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