Recognizes an epitope within aa 20-40 of human, mouse, rat, monkey and dog BimL (~19 kDa), BimEL (~23 kDa), and BimS (~16 kDa). Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region.
交叉反应
犬, 人, 猴, 小鼠, 大鼠
纯度
>95 % (SDS-PAGE)
内毒素水平
<0.1EU/μg purified protein (LAL test, Lonza).
免疫原
Synthetic peptide corresponding to aa 20-40 of mouse BimL.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Liquid
浓度
Lot specific
缓冲液
In PBS containing 0.02 % sodium azide and 25 % glycerol.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Short Term Storage: +4°C Long Term Storage: -20°C Stable for at least 1 year after receipt when stored at -20°C.
有效期
12 months
抗原
BimS, EL, L
别名
BimS/EL/L
背景
Bim belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the Bcl-2 protein family (BCL2, BCL2L1/BCL-X(L) and MCL1) and to act as an apoptotic activator, inducing apoptosis and anoikis. Several variants have been identified. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent.