PDCD1LG2 抗体 (Biotin)
Quick Overview for PDCD1LG2 抗体 (Biotin) (ABIN2743535)
抗原
See all PDCD1LG2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Antibody < span style="font-size: 11.0pt, mso-bidi-font-size: 10.0pt,">ANC8D12 binds to recombinant CD273 and cell surface CD273 expressed on monocyte derived Dendritic cells (moDC), and blocks binding of recombinant CD279 to CD273 in EIA and FACS.
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纯化方法
- Protein A purified
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免疫原
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Recombinant human CD273(B7-DC).
Type of Immunogen: Recombinant protein -
亚型
- IgG2a kappa
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应用备注
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Approved: Block, ELISA, Flo
Usage: Human monocyte derived Dendritic cells were prepared in 8 day culture. 5 x 105 per tube were washed and incubated 45 minutes on ice with 80 μL of antihuman CD273/Biotin at concentration of 10 μg/mL. They were then washed twice and incubated with Streptavidin/R-PE, after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.39 log10 fluorescent units when compared to isotype control Mouse IgG2a/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD273-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 4°C, avoid freeze-thaw cycles.
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- PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))
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别名
- PDCD1LG2 / CD273 / PD-L2
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背景
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Name/Gene ID: PDCD1LG2
Synonyms: PDCD1LG2, B7 dendritic cell molecule, B7-DC, B7DC, BA574F11.2, CD273, Btdc, CD273 antigen, PD-L2, PDCD1 ligand 2, PD-1-ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, PD-1 ligand 2, PDCD1L2, PDL2 -
基因ID
- 80380
抗原
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