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PDCD1LG2 抗体 (Biotin)

This anti-PDCD1LG2 antibody is a 小鼠 单克隆 antibody detecting PDCD1LG2 in FACS, ELISA 和 BR. Suitable for 人.
产品编号 ABIN2743535
发货至: 中国

Quick Overview for PDCD1LG2 抗体 (Biotin) (ABIN2743535)

抗原

See all PDCD1LG2 抗体
PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This PDCD1LG2 antibody is conjugated to Biotin

应用范围

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Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)

克隆位点

ANC8D12
  • 特异性

    Antibody < span style="font-size: 11.0pt, mso-bidi-font-size: 10.0pt,">ANC8D12 binds to recombinant CD273 and cell surface CD273 expressed on monocyte derived Dendritic cells (moDC), and blocks binding of recombinant CD279 to CD273 in EIA and FACS.

    纯化方法

    Protein A purified

    免疫原

    Recombinant human CD273(B7-DC).

    Type of Immunogen: Recombinant protein

    亚型

    IgG2a kappa
  • 应用备注

    Approved: Block, ELISA, Flo

    Usage: Human monocyte derived Dendritic cells were prepared in 8 day culture. 5 x 105 per tube were washed and incubated 45 minutes on ice with 80 μL of antihuman CD273/Biotin at concentration of 10 μg/mL. They were then washed twice and incubated with Streptavidin/R-PE, after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.39 log10 fluorescent units when compared to isotype control Mouse IgG2a/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD273-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store at 4°C, avoid freeze-thaw cycles.
  • 抗原

    PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

    别名

    PDCD1LG2 / CD273 / PD-L2

    背景

    Name/Gene ID: PDCD1LG2

    Synonyms: PDCD1LG2, B7 dendritic cell molecule, B7-DC, B7DC, BA574F11.2, CD273, Btdc, CD273 antigen, PD-L2, PDCD1 ligand 2, PD-1-ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, PD-1 ligand 2, PDCD1L2, PDL2

    基因ID

    80380
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