EpCAM 抗体 (FITC)
Quick Overview for EpCAM 抗体 (FITC) (ABIN2740723)
抗原
See all EpCAM (EPCAM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Antibody ANC8D4 recognizes recombinant human CD326 in EIA, and cell surface human CD326 in FACS.
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纯化方法
- Protein A purified
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免疫原
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Recombinant human CD326(EpCAM).
Type of Immunogen: Recombinant protein -
亚型
- IgG1 kappa
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应用备注
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Approved: ELISA, Flo
Usage: Cultured human SW480 cells were harvested by EDTA. 5 x 105 per tube were pre incubated with 20 μL of 300 μg/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of product at a 1:50 dilution (10 μg/mL). They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.30 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD326 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles
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储存条件
- 4 °C
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储存方法
- Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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别名
- ESA / EPCAM
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背景
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Name/Gene ID: EPCAM
Synonyms: EPCAM, 323/A3, ACSTD1, 17-1A, CD326, EGP, EGP34, Epithelial glycoprotein, GA733-2, HNPCC8, Ep-CAM, ESA, HEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, MIC18, MK-1, MH99, TROP1, TACST-1, TACSTD1, CD326 antigen, CO-17A, DIAR5, EGP-2, EGP314, EGP40, Epithelial glycoprotein 314, HEA125, Ly74, M4S1 -
基因ID
- 4072
抗原
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