EpCAM 抗体 (PE)
Quick Overview for EpCAM 抗体 (PE) (ABIN2740721)
抗原
See all EpCAM (EPCAM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Antibody ANC8D4 recognizes recombinant human CD326 in EIA, and cell surface human CD326 in FACS.
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纯化方法
- Protein A purified
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免疫原
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Recombinant human CD326(EpCAM).
Type of Immunogen: Recombinant protein -
亚型
- IgG1 kappa
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应用备注
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Approved: ELISA, Flo
Usage: Five x 105 cultured human SW480 cells were harvested by trypsiniaztion, washed and pre incubated ~5 minutes with 20 μL of 250 μg/mL human IgG (to block nonspecific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-CD326/R-PE at a dilution factor of 1:50 (10 μg/mL). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.77 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL antiCD326 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 50 mM sodium phosphate, pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 4°C. Do not freeze. Protect from light.
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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别名
- ESA / EPCAM
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背景
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Name/Gene ID: EPCAM
Synonyms: EPCAM, 323/A3, ACSTD1, 17-1A, CD326, EGP, EGP34, Epithelial glycoprotein, GA733-2, HNPCC8, Ep-CAM, ESA, HEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, MIC18, MK-1, MH99, TROP1, TACST-1, TACSTD1, CD326 antigen, CO-17A, DIAR5, EGP-2, EGP314, EGP40, Epithelial glycoprotein 314, HEA125, Ly74, M4S1 -
基因ID
- 4072
抗原
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