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EpCAM 抗体 (Biotin)

The Biotin-conjugated 小鼠 单克隆 anti-EpCAM antibody (Clone ANC8D4) (ABIN2740720) specifically detects EpCAM in FACS 和 ELISA. The antibody is reactive with 人 samples.
产品编号 ABIN2740720
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
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Quick Overview for EpCAM 抗体 (Biotin) (ABIN2740720)

抗原

See all EpCAM (EPCAM) 抗体
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This EpCAM antibody is conjugated to Biotin

应用范围

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Flow Cytometry (FACS), ELISA

克隆位点

ANC8D4
  • 特异性

    Antibody ANC8D4 recognizes recombinant human CD326 in EIA, and cell surface human CD326 in FACS.

    纯化方法

    Protein A purified

    免疫原

    Recombinant human CD326(EpCAM).

    Type of Immunogen: Recombinant protein

    亚型

    IgG1 kappa
  • 应用备注

    Approved: ELISA, Flo

    Usage: Cultured human SW480 cells were harvested by EDTA. 5 x 105 per tube were washed and pre incubated ~5 minutes with 20 μL human IgG at 250 μg/mL (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 μL of anti-human CD326/Biotin at concentration of 5 μg/mL. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.93 log10 fluorescent units when compared to an Isotype control Mouse IgG1/Biotin. Binding was blocked when cells were pre incubated with unlabeled antihuman CD326 mAb (#126-020). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store at 4°C, avoid freeze-thaw cycles.
  • 抗原

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    别名

    ESA / EPCAM

    背景

    Name/Gene ID: EPCAM

    Synonyms: EPCAM, 323/A3, ACSTD1, 17-1A, CD326, EGP, EGP34, Epithelial glycoprotein, GA733-2, HNPCC8, Ep-CAM, ESA, HEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, MIC18, MK-1, MH99, TROP1, TACST-1, TACSTD1, CD326 antigen, CO-17A, DIAR5, EGP-2, EGP314, EGP40, Epithelial glycoprotein 314, HEA125, Ly74, M4S1

    基因ID

    4072
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