CD8 alpha 抗体 (Cy7,PE)
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北京 101111
Quick Overview for CD8 alpha 抗体 (Cy7,PE) (ABIN2739737)
抗原
See all CD8 alpha (CD8A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Mouse CD8.
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纯化方法
- Protein G purified
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免疫原
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Mouse thymocytes/splenocytes.
Type of Immunogen: Cells -
亚型
- IgG2a kappa
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应用备注
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Approved: ELISA, Flo
Usage: Reagent was tested for binding to ACKlysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 μL of 300 μg/mL Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-Mouse CD8/PE-Cy7 at a 1:50 dilution (5 μg/mL). They were then washed three times, fixed and analyzed by FACS. An 8.3 % sub population of the cells stained positive with a mean shift of 1.4 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-mouse CD8 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 50 mM sodium phosphate, pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 4°C. Do not freeze. Protect from light.
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- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
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别名
- CD8A / CD8 alpha
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背景
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Name/Gene ID: CD8A
Synonyms: CD8A, CD8a molecule, CD8a antigen, OKT8 T-cell antigen, Leu2 T-lymphocyte antigen, T8 T-cell antigen, T cell co-receptor, p32, CD8, Leu2, T-cell antigen Leu2 -
基因ID
- 925
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途径
- TCR Signaling
抗原
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