Actin 抗体 (N-Term)
Quick Overview for Actin 抗体 (N-Term) (ABIN2738124)
抗原
See all Actin 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- Human Actin
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纯化方法
- Protein G purified
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免疫原
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An N-Terminal decapeptide of the alpha smooth muscle isoform.
Type of Immunogen: Synthetic peptide -
亚型
- IgG2a kappa
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应用备注
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Approved: ELISA, Flo (0.5 - 1 μg/10 x106), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)
Usage: Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle. however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin. No special pretreatment is required for the immunohistochemical staining of FFPE tissue. ELISA: Order BSA-free format. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 1 month or -20°C for longer. Avoid freeze/thaw cycles.
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- Actin
抗原
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