MYOD1 抗体
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北京 101111
Quick Overview for MYOD1 抗体 (ABIN272324)
抗原
See all MYOD1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified
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免疫原
- Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.
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亚型
- IgG2a
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应用备注
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ELISA. Western Blot: 1 μg/mL. Immunofluorescence. Electron microscopy. Immunoprecipitation: 2 μg/mg of protein lysate. Immunohistochemistry on Frozen and Paraffin Sections: 2-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- Phosphate buffered saline with 0.08 % Sodium Azide as preserative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MYOD1 (Myogenic Differentiation 1 (MYOD1))
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别名
- MYOD1
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背景
- Recognizes a phosphoprotein of 45 kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.Synonyms: BHLHC1, Class C basic helix-loop-helix protein 1, MYF3, MYOD, Myoblast determination protein 1, Myogenic factor 3
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基因ID
- 10090
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UniProt
- P10085
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
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