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MYOD1 抗体

This anti-MYOD1 antibody is a 小鼠 单克隆 antibody detecting MYOD1 in WB, IF, IP, IHC (p), IHC (fro) 和 EIA. Suitable for 人, 小鼠, 大鼠 和 小鸡.
产品编号 ABIN272324
发货至: 中国

Quick Overview for MYOD1 抗体 (ABIN272324)

抗原

See all MYOD1 抗体
MYOD1 (Myogenic Differentiation 1 (MYOD1))

适用

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人, 小鼠, 大鼠, 小鸡

宿主

  • 90
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小鼠

克隆类型

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单克隆

标记

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This MYOD1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

克隆位点

5-2F
  • 纯化方法

    Purified

    免疫原

    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.

    亚型

    IgG2a
  • 应用备注

    ELISA. Western Blot: 1 μg/mL. Immunofluorescence. Electron microscopy. Immunoprecipitation: 2 μg/mg of protein lysate. Immunohistochemistry on Frozen and Paraffin Sections: 2-4 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 缓冲液

    Phosphate buffered saline with 0.08 % Sodium Azide as preserative

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    MYOD1 (Myogenic Differentiation 1 (MYOD1))

    别名

    MYOD1

    背景

    Recognizes a phosphoprotein of 45 kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.Synonyms: BHLHC1, Class C basic helix-loop-helix protein 1, MYF3, MYOD, Myoblast determination protein 1, Myogenic factor 3

    基因ID

    10090

    UniProt

    P10085

    途径

    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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