YWHAE 抗体
(2 references)Quick Overview for YWHAE 抗体 (ABIN272265)
抗原
See all YWHAE 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of 14-3-3 ε protein. (region surrounding Asp238)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography using epitope-specific immunogen
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anti-14-3-3 epsilon (YWHAE) (AA 1-255) antibody
YWHAE 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) antibodyYWHAE 适用: 人 WB, IHC (p) 宿主: 兔 Monoclonal 2E2 unconjugated
anti-14-3-3 epsilon (YWHAE) antibodyYWHAE 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal CPTC-YWHAE-1 unconjugated
anti-14-3-3 epsilon (YWHAE) (C-Term) antibodyYWHAE 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) (C-Term) antibodyYWHAE 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 绵羊, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) (Middle Region) antibodyYWHAE 适用: 小鼠, 斑马鱼, 豚鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) (AA 151-255) antibodyYWHAE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) (C-Term) antibodyYWHAE 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) (C-Term) antibodyYWHAE 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 epsilon (YWHAE) (AA 1-255) antibodyYWHAE 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8° for one month or (in aliquots) at -20 °C for longer.
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: "In Ovo injection of betaine affects hepatic cholesterol metabolism through epigenetic gene regulation in newly hatched chicks." in: PLoS ONE, Vol. 10, Issue 4, pp. e0122643, (2016) (PubMed).
: "Helicobacter pylori infection combined with DENA revealed altered expression of p53 and 14-3-3 isoforms in Gulo-/- mice." in: Chemico-biological interactions, Vol. 206, Issue 2, pp. 143-52, (2013) (PubMed).
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: "In Ovo injection of betaine affects hepatic cholesterol metabolism through epigenetic gene regulation in newly hatched chicks." in: PLoS ONE, Vol. 10, Issue 4, pp. e0122643, (2016) (PubMed).
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- YWHAE (14-3-3 epsilon (YWHAE))
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别名
- 14-3-3 Protein epsilon
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背景
- 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.Synonyms: 14-3-3E, YWHAE
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分子量
- approx. 29 kDa
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基因ID
- 7531
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NCBI登录号
- NP_006752
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UniProt
- P62258
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途径
- Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase
抗原
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