PFKFB1 抗体
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Quick Overview for PFKFB1 抗体 (ABIN272241)
抗原
See all PFKFB1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of PFKFB1/4 protein. (region surrounding Glu349)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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anti-6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1) antibody
PFKFB1 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1) (AA 1-471) antibodyPFKFB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1) (C-Term) antibodyPFKFB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1) (C-Term) antibodyPFKFB1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1) (AA 259-289) antibodyPFKFB1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4038 unconjugated
anti-6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1) (AA 13-43), (N-Term) antibodyPFKFB1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB4035 unconjugated
anti-6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1) (AA 1-100) antibodyPFKFB1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1) (AA 335-384) antibodyPFKFB1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 斑马鱼, 兔, Bat, 猴, Pig, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1) (C-Term) antibodyPFKFB1 适用: 人, Cow, 犬, 豚鼠, 马, 斑马鱼, Bat, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1) (Internal Region) antibodyPFKFB1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE!
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- PFKFB1 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1))
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别名
- PFKFB1
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背景
- PFKFB (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase) is a bifunctional enzyme, having both kinase and phosphatase activities residing on the same enzyme subunit but having distinct active sites. PFKFB regulates the steady-state concentration of fructose-2,6-bisphosphate, a potent activator of a key regulatory enzyme of glycolysis, phosphofructokinase. To date, four PFKFB isozymes (PFKFB 1-4) have been described, which show differences in their tissue distribution and kinetic properties in response to allosteric effectors and hormonal signals. Among the PFKFB's PFKFB3 has the highest kinase:phosphatase ratio, in part because it lacks the characteristic serine phosphorylation site near the N-terminal that down-modulates kinase activity. PFKFB3 was first described in the rapidly growing placenta. The glucolitic rate in placenta is accelerated by anoxia and by maternal diabetes. Cancer cells maintain a high glycolytic rate even in the presence of oxygen, a phenomenon known as the Warburg effect. The glycolytic rate in the placenta, another fast-growing tissue, is accelerated by anoxia and by maternal diabetes.Synonyms: 6-biphosphatase 1, 6-phosphofructo-2-kinase/fructose-2, F6PK, PFK-2 liver, PFK/FBPase 1, PFRX
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分子量
- approx. 54 kDa
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基因ID
- 5207
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NCBI登录号
- NP_002616
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UniProt
- P16118
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途径
- Regulation of Carbohydrate Metabolic Process
抗原
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