CEBPE 抗体
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北京 101111
Quick Overview for CEBPE 抗体 (ABIN272237)
抗原
See all CEBPE 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of C/EBP-epsilon protein. (region surrounding Lys220)
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交叉反应 (详细)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography using epitope-specific immunogen.
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应用备注
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ELISA: 1/10000approx. 1/20000. Western blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CEBPE (CCAAT/enhancer Binding Protein (C/EBP), epsilon (CEBPE))
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别名
- CEBPE
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背景
- The transcription factor C/EBP alpha (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma,C/EBP delta and C/EBP epsilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.Synonyms: C/EBP epsilon, CCAAT/enhancer-binding protein epsilon
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分子量
- approx. 30 kDa
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基因ID
- 1053
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NCBI登录号
- NP_001796
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UniProt
- Q15744
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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