MED1 抗体
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北京 101111
Quick Overview for MED1 抗体 (ABIN272236)
抗原
See all MED1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of TRAP220 protein. (region surrounding Pro697)
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交叉反应 （详细）
- Species reactivity (tested):Human, Mouse.
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纯化方法
- Affinity Chromatography using epitope-specific immunogen.
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anti-Mediator Complex Subunit 1 (MED1) (AA 1550-1581) antibody
MED1 适用: 人, 小鼠 WB, IHC, IP, ICC, FACS 宿主: 兔 Monoclonal BLR037F unconjugated Recombinant Antibody
anti-Mediator Complex Subunit 1 (MED1) (pThr1457) antibodyMED1 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (AA 1391-1490) antibodyMED1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2A2 unconjugated
anti-Mediator Complex Subunit 1 (MED1) (C-Term) antibodyMED1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (C-Term) antibodyMED1 适用: 人, 小鼠 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (C-Term) antibodyMED1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (AA 1-212) antibodyMED1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (AA 878-1031) antibodyMED1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (AA 1531-1581) antibodyMED1 适用: 人, 小鼠 WB, IP, IHC (fp) 宿主: 山羊 Polyclonal unconjugated
anti-Mediator Complex Subunit 1 (MED1) (C-Term) antibodyMED1 适用: 人, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MED1 (Mediator Complex Subunit 1 (MED1))
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别名
- MED1
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背景
- In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signaling between transcriptional activators and initiation factors. These complexes include the thyroid hormone receptor-associated protein (TRAP) complex, which interacts with thyroid receptors (TR), vitamin D receptors and other steroid receptors to facilitate hormone induced transcriptional activation. The TRAP complex consists of numerous proteins ranging in size from 20 to 240 kDa, including TRAP95, TRAP100, TRAP150, TRAP220 and TRAP230, that are characterized by the presence of a nuclear receptor recognition motif which mediates the liganddependent binding of TRAP proteins to the nuclear receptors. TRAP220 and TRAP100 are widely expressed and most abundantly detected in skeletal muscle, heart and placenta. TRAP95, TRAP150 and TRAP230 facilitate TR induced transcription by associating with an additional transcriptional coactivating complex SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes.Synonyms: ARC205, Activator-recruited cofactor 205 kDa component, CRSP1, CRSP200, DRIP205, DRIP230, Mediator complex subunit 1, PBP, PPARBP, PPARGBP, Peroxisome proliferator-activated receptor-binding protein, RB18A, TRAP220, TRIP2, Thyroid hormone receptor-associated protein complex 220 kDa component, Thyroid receptor-interacting protein 2, Vitamin D receptor-interacting protein complex component DRIP205, p53 regulatory protein RB18A
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分子量
- approx. 170 kDa
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基因ID
- 5469
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NCBI登录号
- NP_004765
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UniProt
- Q15648
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途径
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
抗原
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