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CEBPE 抗体

The 兔 多克隆 anti-CEBPE antibody is suitable to detect CEBPE in samples from 人, 大鼠 和 小鼠. It has been validated for WB, IF 和 IHC (p).
产品编号 ABIN272198
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CEBPE 抗体 (ABIN272198)

抗原

See all CEBPE 抗体
CEBPE (CCAAT/enhancer Binding Protein (C/EBP), epsilon (CEBPE))

适用

  • 49
  • 13
  • 13
  • 1
人, 大鼠, 小鼠

宿主

  • 44
  • 5

克隆类型

  • 45
  • 4
多克隆

标记

  • 26
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
This CEBPE antibody is un-conjugated

应用范围

  • 30
  • 25
  • 12
  • 10
  • 4
  • 2
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    This antibody detects endogenous levels of C/EBP-epsilon protein. (region surrounding Ala217)

    交叉反应 (详细)

    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

    纯度

    > 95 % pure by SDS-PAGE
  • 应用备注

    ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1,0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    DO NOT FREEZE!

    储存条件

    4 °C

    储存方法

    Store the antibody undiluted at 2-8 °C.
  • 抗原

    CEBPE (CCAAT/enhancer Binding Protein (C/EBP), epsilon (CEBPE))

    别名

    CEBPE

    背景

    The transcription factor C/EBP alpha (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma,C/EBP delta and C/EBP epsilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.Synonyms: C/EBP epsilon, CCAAT/enhancer-binding protein epsilon

    分子量

    approx. 28 kDa

    基因ID

    1053

    NCBI登录号

    NP_001796

    UniProt

    Q15744

    途径

    Cellular Response to Molecule of Bacterial Origin
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