PFKL 抗体
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北京 101111
Quick Overview for PFKL 抗体 (ABIN272176)
抗原
See all PFKL 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of PFK-B protein. (region surrounding Glu724)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography using epitope-specific immunogen.
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anti-Phosphofructokinase, Liver (PFKL) (Center) antibody
PFKL 适用: 人, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphofructokinase, Liver (PFKL) (AA 229-479) antibodyPFKL 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphofructokinase, Liver (PFKL) (AA 700-780) antibodyPFKL 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphofructokinase, Liver (PFKL) (AA 669-699), (C-Term) antibodyPFKL 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB03903 unconjugated
anti-Phosphofructokinase, Liver (PFKL) (Middle Region) antibodyPFKL 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 绵羊, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Phosphofructokinase, Liver (PFKL) (AA 371-399) antibodyPFKL 适用: 人 WB 宿主: 兔 Polyclonal RB6851 unconjugated
anti-Phosphofructokinase, Liver (PFKL) (C-Term) antibodyPFKL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphofructokinase, Liver (PFKL) (AA 441-540) antibodyPFKL 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A9 unconjugated
anti-Phosphofructokinase, Liver (PFKL) (C-Term) antibodyPFKL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphofructokinase, Liver (PFKL) (C-Term) antibodyPFKL 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PFKL (Phosphofructokinase, Liver (PFKL))
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别名
- PFKL
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背景
- Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).Synonyms: 6-phosphofructokinase liver, PFK-B, Phosphofructokinase 1, Phosphohexokinase
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分子量
- approx. 85 kDa
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基因ID
- 5211
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NCBI登录号
- NP_002617
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UniProt
- P17858
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途径
- Negative Regulation of Hormone Secretion, Warburg Effect
抗原
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