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ATF7 抗体

The 兔 多克隆 anti-ATF7 antibody is suitable to detect ATF7 in samples from 人 和 小鼠. It has been validated for WB, IF 和 IHC (p).
产品编号 ABIN272174
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for ATF7 抗体 (ABIN272174)

抗原

See all ATF7 抗体
ATF7 (Activating Transcription Factor 7 (ATF7))

适用

  • 48
  • 30
  • 19
  • 8
  • 8
  • 7
  • 7
  • 6
  • 5
  • 3
  • 3
  • 2
人, 小鼠

宿主

  • 45
  • 4
  • 1

克隆类型

  • 40
  • 10
多克隆

标记

  • 29
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
This ATF7 antibody is un-conjugated

应用范围

  • 26
  • 9
  • 8
  • 8
  • 6
  • 5
  • 2
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    This antibody detects endogenous levels of ATF-7 protein. (region surrounding Phe74)

    交叉反应 (详细)

    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.

    纯化方法

    Affinity Chromatography using epitope-specific immunogen.
  • 应用备注

    ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    ATF7 (Activating Transcription Factor 7 (ATF7))

    别名

    ATF7

    背景

    Eukaryotic gene transcription is regulated by sequence-specific transcription factors, which bind modular cis acting promoter and enhancer elements. The cAMP response element (CRE) consists of the palindromic octanucleotide TGACGTCA. There are several CRE binding proteins within the ATF/CREB family, including CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. A novel basic leucine zipper (bZIP) protein, designated ATF-7, is closely related to members of the ATF/CREB family of bZIP proteins, with highest homology to ATF-4. ATF-7 physically interacts with the PRL-1 protein-tyrosine phosphatase (PTPase), which is a predominately nuclear, farnesylated PTPase. ATF-7 homodimers bind specifically to CRE elements. ATF-7 is expressed in a number of different tissues and is expressed in association with differentiation. ATF-7 and PRL-1 interact with each other through the bZIP region of ATF-7 and the phosphatase domain of PRL-1. In addition, PRL-1 is able to dephosphorylate ATF-7 in vitro.Synonyms: ATF-7, ATF-A, ATFA, Activating transcription factor 7, Cyclic AMP-dependent transcription factor ATF-7, cAMP-dependent transcription factor ATF-7

    分子量

    approx. 35, 53 kDa

    基因ID

    11016

    NCBI登录号

    NP_001123531

    UniProt

    P17544
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