UBTF 抗体
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北京 101111
Quick Overview for UBTF 抗体 (ABIN272172)
抗原
See all UBTF 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of UBF-1 protein. (region surrounding Glu536)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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anti-Upstream Binding Transcription Factor, RNA Polymerase I (UBTF) (AA 551-650) antibody
UBTF 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 6B6 unconjugated
anti-Upstream Binding Transcription Factor, RNA Polymerase I (UBTF) (AA 551-650) antibodyUBTF 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 1A2 unconjugated
anti-Upstream Binding Transcription Factor, RNA Polymerase I (UBTF) (AA 551-650) antibodyUBTF 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 6C6 unconjugated
anti-Upstream Binding Transcription Factor, RNA Polymerase I (UBTF) (Internal Region) antibodyUBTF 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Upstream Binding Transcription Factor, RNA Polymerase I (UBTF) (AA 551-651) antibodyUBTF 适用: 人, 大鼠 WB, ELISA, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 1A2 unconjugated
anti-Upstream Binding Transcription Factor, RNA Polymerase I (UBTF) (AA 551-651) antibodyUBTF 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 2D8 unconjugated
anti-Upstream Binding Transcription Factor, RNA Polymerase I (UBTF) (AA 200-440) antibodyUBTF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Upstream Binding Transcription Factor, RNA Polymerase I (UBTF) (pSer484) antibodyUBTF 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Upstream Binding Transcription Factor, RNA Polymerase I (UBTF) (Internal Region) antibodyUBTF 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Upstream Binding Transcription Factor, RNA Polymerase I (UBTF) antibodyUBTF 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB1555 unconjugated Recombinant Antibody
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应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE!
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))
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别名
- UBTF / UBF
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背景
- The transcription of ribosomal RNA genes by RNA polymerase I (Pol I) is tightly coordinated with the growth state of the cell. In addition to Pol I, transcription of ribosomal genes requires the trans-activating factor UBF (upstream binding factor). UBF functions by binding to DNA elements within the RNA gene promoter and enhancer regions and directly associating with Pol I, tethering it to the promoter complex. Two UBF proteins, of 97 kDa and 94 kDa, arise from the same gene as a result of alternative mRNA splicings. UBF activity is regulated by several dependent casein kinase II phosphorylates at the carboxy terminus of UBF on serine residues. The retinoblastoma susceptibility gene product, Rb, when not bound to E2F family members, inhibits UBF activity. Expression of RNA may also be negatively regulated by the 70 kDa and 86 kDa Ku antigens.Synonyms: Autoantigen NOR-90, Nucleolar transcription factor 1, UBF-1, UBF1, Upstream-binding factor 1
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分子量
- approx. 90 kDa
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基因ID
- 7343
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NCBI登录号
- NP_001070151
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UniProt
- P17480
抗原
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