EpCAM 抗体
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Quick Overview for EpCAM 抗体 (ABIN272170)
抗原
See all EpCAM (EPCAM) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Ep-CAM protein. (region surrounding His150)
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交叉反应 (详细)
- Species reactivity (tested):Human.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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应用备注
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ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE!
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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别名
- CD326 / EPCAM / TACSTD1
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背景
- The epithelial cell adhesion molecule Ep-CAM, which is also design atedtumor-associated calcium signal transducer 1 and MK-1, is a monomeric membrane glycoprotein that is expressed in most normal human epithelium and in most carcinomas. The human Ep-CAM gene encodes a 314 amino acid protein that is expressed as two forms, a 40 kDa major form and a 42 kDa minor form, which are reduced to 35 kDa upon treatment with the aminoglycosylation inhibitor tunicamycin. Ep-CAM is overexpressed in a variety of carcinomas and is, therefore, a potential target for the visualization and therapy of human solid tumours. Ep-CAM contains an extracellular domain containing two epidermal growth factor-like repeats, followed by a cysteinepoor region, which are necessary for the adhesion properties of the molecule.Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
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分子量
- approx. 35 kDa
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基因ID
- 4072
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NCBI登录号
- NP_002345
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UniProt
- P16422
抗原
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