HNRNPL 抗体
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北京 101111
Quick Overview for HNRNPL 抗体 (ABIN272168)
抗原
See all HNRNPL 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of hnRNP L protein. (region surrounding Gly58)
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交叉反应 (详细)
- Species reactivity (tested):Human, Mouse.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE!
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- HNRNPL (Heterogeneous Nuclear Ribonucleoprotein L (HNRNPL))
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别名
- HnRNP-L / HNRNPL
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背景
- Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing and mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II). There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the majorconstituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm. hnRNP I, also designated polypyrimidine tract-binding protein (PTB) and its homolog hnRNP L bind to the 3' end of introns to modulate alternative splicing mechanisms of pre-mRNAs in normal cells and the translation of several viruses, including hepatitis C virus (HCV). The human hnRNP I gene maps to chromosome 19p13.3 and encodes a 57 kDa protein that is localized in the nucleoplasm. hnRNP L has a molecular mass of 68 kDa and like hnRNP I, is also localized in the nucleoplasm.Synonyms: HNRPL, Heterogeneous nuclear ribonucleoprotein L, P/OKcl.14
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分子量
- approx. 60 kDa
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基因ID
- 3191
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NCBI登录号
- NP_001005335
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UniProt
- P14866
抗原
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