HARS1/Jo-1 抗体
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北京 101111
Quick Overview for HARS1/Jo-1 抗体 (ABIN272164)
抗原
See all HARS1/Jo-1 (HARS1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of HisRS protein. (region surrounding Glu492)
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交叉反应 （详细）
- Species reactivity (tested):Human, Mouse.
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纯化方法
- Affinity Chromatography using epitope-specific immunogen.
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anti-Histidyl-tRNA Synthetase (HARS1) (AA 1-96) antibody
HARS1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4D4 unconjugated
anti-Histidyl-tRNA Synthetase (HARS1) (AA 1-509) antibodyHARS1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Histidyl-tRNA Synthetase (HARS1) (AA 1-96) antibodyHARS1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C8 unconjugated
anti-Histidyl-tRNA Synthetase (HARS1) (AA 1-180) antibodyHARS1 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Histidyl-tRNA Synthetase (HARS1) antibodyHARS1 适用: 人 WB, IP, ICC, FACS, DB 宿主: 小鼠 Monoclonal HARSA6 unconjugated
anti-Histidyl-tRNA Synthetase (HARS1) (C-Term) antibodyHARS1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histidyl-tRNA Synthetase (HARS1) (AA 1-96) antibodyHARS1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Histidyl-tRNA Synthetase (HARS1) (C-Term) antibodyHARS1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Histidyl-tRNA Synthetase (HARS1) (AA 49-78), (N-Term) antibodyHARS1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB14816 unconjugated
anti-Histidyl-tRNA Synthetase (HARS1) (C-Term) antibodyHARS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- HARS1/Jo-1 (HARS1) (Histidyl-tRNA Synthetase (HARS1))
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别名
- Histidyl-tRNA Synthetase / HARS
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背景
- Histidyl-tRNA synthetase (HisRS, Jo-1) is an aminoacyl-tRNA synthetase that charges transfer RNAs (tRNAs) with a cognate histidine residue to produce histidyl-tRNA. Histidyl-tRNA is essential for the incorporation of histidine into proteins. Systemic autoimmune diseases lead to aminoacyl-tRNA synthetase autoantibodies that are directed against self structures. HisRS is an antigen in systemic autoimmune diseases such as rheumatic arthritis or polymyositis with associated interstitial lung disease, thrombocytopenia and Raynaud's phenomenon (vasospastic attacks that cause blood vessels to constrict). HisRS autoantibodies are unlike anti-synthetase antibodies because they bind the nonlinear, exposed epitopes on native HisRS when the enzyme is complexed to tRNA(His).Synonyms: HRS, HisRS, Histidine-tRNA ligase, JO1, Jo-1 antigen
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分子量
- approx. 60 kDa
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基因ID
- 3035
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NCBI登录号
- NP_002100
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UniProt
- P12081
抗原
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