ETF1 抗体
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- 抗原 See all ETF1 抗体
- ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ETF1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS)
- 产品特性
- Homo sapiens eukaryotic translation termination factor 1 (ETF1)
- 纯化方法
- Purified from mouse ascites fluids by affinity chromatography
- 免疫原
- Full length human recombinant protein of human ETF1 (NP_004721) produced in HEK293T cell.
- 克隆位点
- 2E3
- 亚型
- IgG2a
- Top Product
- Discover our top product ETF1 Primary Antibody
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- 应用备注
- WB 1:500, FLOW 1:100,
- 说明
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The concentration of the product may vary between diferrent lots.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5-1.0 mg/mL
- 缓冲液
- PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))
- 别名
- ETF1 (ETF1 产品)
- 背景
- Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).
- 分子量
- 48.9 kDa
- 基因ID
- 2107
- NCBI登录号
- NM_004730
- HGNC
- 2107
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