PPP1R12A 抗体
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北京 101111
Quick Overview for PPP1R12A 抗体 (ABIN272043)
抗原
See all PPP1R12A 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of MYPT1 protein. (region surrounding Arg847)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography using epitope-specific immunogen.
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anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 1-200) antibody
PPP1R12A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (C-Term) antibodyVerified PPP1R12A 适用: 人 ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 1-40), (N-Term) antibodyPPP1R12A 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (C-Term) antibodyPPP1R12A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pThr696) antibodyPPP1R12A 适用: 人, 大鼠 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pThr853) antibodyPPP1R12A 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 710-970) antibodyPPP1R12A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 481-514), (Ser507) antibodyPPP1R12A 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB56872 unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pThr853) antibodyPPP1R12A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pSer696) antibodyPPP1R12A 适用: 人, 小鼠, 大鼠, 斑马鱼, 小鸡, Pig WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/5000-1/10000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
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别名
- PPP1R12A / MYPT1
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背景
- Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.Synonyms: MBS, Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase 1 regulatory subunit 12A, Protein phosphatase myosin-binding subunit
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分子量
- approx. 140 kDa
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基因ID
- 4659
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NCBI登录号
- NP_001137357
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UniProt
- O14974
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途径
- M Phase
抗原
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