EIF4E 抗体
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- 抗原 See all EIF4E 抗体
- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This EIF4E antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS)
- 产品特性
- Homo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1
- 纯化方法
- Purified from mouse ascites fluids by affinity chromatography
- 免疫原
- Full length human recombinant protein of human EIF4E (NP_001959) produced in HEK293T cell.
- 克隆位点
- 6C11
- 亚型
- IgG1
- Top Product
- Discover our top product EIF4E Primary Antibody
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- 应用备注
- WB 1:500~2000, FLOW 1:100
- 说明
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The concentration of the product may vary between diferrent lots.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5-1.0 mg/mL
- 缓冲液
- PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
- 别名
- EIF4E (EIF4E 产品)
- 背景
- All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).
- 分子量
- 24.9 kDa
- 基因ID
- 1977
- NCBI登录号
- NM_001968
- HGNC
- 1977
- 途径
- BCR Signaling
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