Actin 抗体 (pan)
Quick Overview for Actin 抗体 (pan) (ABIN271986)
抗原
See all Actin 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- pan
-
特异性
- This antibody detects endogenous levels ofActin-pan (α/β/γ) protein (region surrounding Gln49)
-
交叉反应 （详细）
- Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.
-
纯化方法
- Affinity-chromatography
-
纯度
- > 95 % (by SDS-PAGE)
-
-
anti-Actin antibody
适用: 植物 WB 宿主: 小鼠 Monoclonal Q30 unconjugated
anti-Actin (AA 165-260) antibody适用: 人, 大鼠, 小鼠, 兔, 犬 WB, ELISA, IHC (p), IF (cc), IF (p), FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Actin antibody适用: 人, 大鼠, 小鼠, 小鸡, 犬, Cow, Pig, 豚鼠 WB, ELISA, IHC (p), IF (cc), IF (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Actin (C-Term) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Actin antibody适用: Oryza sativa, Barley, Arabidopsis, Nicotiana tabacum WB 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 367-377) antibody适用: 人, 大鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 21-70) antibody适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 38-67), (N-Term) antibody适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin (AA 189-218) antibody适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 1,0 mg/mL
-
缓冲液
- PBS, pH 7.2, 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
-
- Actin
-
背景
- Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Be aware when using beta actin antibodies as loading controls that although beta actin is normally the best protein to use as a loading control, under some conditions its amounts can vary between samples. In particular, expression of actin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) "in cells undergoing adipose differentiation actin synthesis decreases by 90 % ".
-
分子量
- approx. 45 kDa
抗原
-
